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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Updated: Jun 29, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

SingmiR:一个单细胞miRNA对齐和分析工具.

Annika Engel¹, Shusruto Rishik¹, Pascal Hirsch¹

¹Chair for Clinical Bioinformatics, Saarland University, 66123 Saarbrücken, Germany.

Nucleic acids research

|April 4, 2024

概括

SingmiR提供了一个用户友好的Web服务器,用于处理单细胞miRNA测序数据. 这种工具加速了对单个细胞中微RNA调节的分析,有助于生物学发现.

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Last Updated: Jun 29, 2025

mirMachine: A One-Stop Shop for Plant miRNA Annotation

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Published on: May 1, 2021

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

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Published on: September 16, 2019

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

科学领域:

基因组学和分子生物学
生物信息学和计算生物学

背景情况:

单细胞RNA测序 (RNA-seq) 已经推进了细胞生物学,但在单细胞水平上,微RNA (miRNA) 调节过程在很大程度上未被探索.
分析单细胞miRNA测序 (miRNA-seq) 数据需要先进的计算技能,并且缺乏专门的,用户友好的工作流.

研究的目的:

为了介绍SingmiR,一个新的Web服务器,旨在快速预处理和定量人类单细胞miRNA-seq数据.
为生物学家提供一种可访问的计算工具,以促进对单个细胞中miRNA表达和调节的分析.

主要方法:

开发了一个Web服务器,实现单细胞miRNA-seq数据的全面工作流.
包括读取修剪,自动质量控制和功能正常化等功能.
集成的标准和高级分析模块,如尺寸缩小,热图和微分表达式分析.

主要成果:

SingmiR 能够有效地从单细胞样本中预处理和量化人类miRNA.
服务器提供自动化质量控制报告和规范化计数文件.
为中小型数据集提供各种分析工具,以加快单细胞miRNA-seq协议的原型设计.

结论:

SingmiR解决了对单细胞miRNA-seq数据分析的专业化,用户友好的计算工具的需求.
该平台旨在加快对单细胞水平上miRNAs转录后基因调节的研究.
SingmiR是免费访问的,促进更广泛的采用和在该领域的进步.