解读精子和卵巢微环境之间的动态相互作用通过集成的多omics方法在活体的Sebastes schlegeliilii
Rui Li1, Jiangbo Qu1, Kai Yan1
1MOE Key Laboratory of Marine Genetics and Breeding/Key Laboratory of Tropical Aquatic Germplasm of Hainan Province, Sanya Oceanographic Institution, College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
Development (Cambridge, England)
|April 4, 2024
概括
精子动物通过分子变化适应卵巢微环境,包括蛋白质和糖重塑. 这项研究揭示了像ZPB2a和GSTM3这样的特定蛋白质如何促进精子的生存和运动,以便在鱼类中成功繁殖.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 鱼类的繁殖 鱼类的繁殖
背景情况:
- 卵巢微环境对于生殖成功至关重要.
- 精子-卵巢相互作用的分子机制仍然不太清楚.
研究的目的:
- 阐明黑岩鱼 (Sebastes schlegelii) 精子-卵巢微环境相互作用的分子机制.
- 了解女性生殖道内的精子适应和生存策略.
主要方法:
- 采用了综合的多主题方法.
- 分析包括不同存储阶段的卵巢和精子样本的蛋白质和糖质分析.
主要成果:
- 卵巢补充蛋白和模式识别受体在交配后的早期被上调.
- 精子细胞的表面糖基因被重塑,精子通过托和酸盐抑制补充蛋白来适应.
- 细胞结蛋白在受精前被上调;ZPB2a和GSTM3的相互作用被确定为精子的生存和运动.
结论:
- 确定了调节卵巢微环境中的精子适应和生存的关键分子相互作用.
- 揭示了一种涉及ZPB2a和GSTM3的新型机制,用于内部受精中的精子功能.
相关概念视频
01:38Fertilization
70.9K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
70.9K
01:41Spermatogenesis
102.4K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.4K