Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

5.3K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
5.3K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Clinical and molecular characterization of TCF12 variants in an Asian pediatric cohort with craniosynostosis.

BMC medical genomics·2026
Same author

Two-coordinate Cu(I) sites in ZIF-derived porous carbon enable high CO working capacity for CO/N₂ separation.

Nature communications·2026
Same author

Residue-Driven Degradation and Its Stabilization in NaCl-Assisted WS<sub>2</sub> with 3R Stacking Enrichment.

ACS applied materials & interfaces·2026
Same author

CREAT: A CRISPR-Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Atomic-Scale Full-Size Engineering of Platinum Nanozymes Enables High-Efficiency Catalytic Therapy.

ACS nano·2026
Same author

Predictive value of NRI, NLR, PLR and LMR for therapeutic efficacy and prognosis in advanced rectal cancer patients treated with PD-1/PD-L1 immunotherapy.

Frontiers in surgery·2026

相关实验视频

Updated: Jun 29, 2025

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K

双顶螺旋接口模拟器

Tianxiong Mi1, Zhe Gao1, Zeynep Mituta2

  • 1Department of Chemistry, Texas A & M University, Box 30012, College Station, Texas 77842, United States.

Journal of the American Chemical Society
|April 4, 2024
PubMed
概括

研究人员开发了双顶螺旋模拟器来破坏蛋白相互作用. 这些稳定,穿透细胞的探针能够抵抗降解, 为药物设计提供了新的方法.

科学领域:

  • 药物化学
  • 生物分子工程
  • 类科学

背景情况:

  • 破坏蛋白质之间的相互作用是关键的治疗策略.
  • 螺旋式探针可以模仿蛋白质接口,但在稳定性和传递方面面临挑战.
  • 现有的方法主要采用侧链链接,在终端螺旋上只有有限的成功.

研究的目的:

  • 开发一种可通用的方法来创建结构稳定的螺旋模拟.
  • 研究双顶模剂在治疗应用中的潜力.
  • 为了比较双顶模仿剂与传统合的稳定性和有效性.

主要方法:

  • 双循环螺旋模剂的设计和合成.
  • 使用生物物理技术评估构造稳定性.
  • 对标蛋白 (A和E环素) 的结合亲和度的评估.
  • 细胞吸收研究和血清蛋白质溶解耐药性测试.

主要成果:

  • 双顶系统表现出明确的螺旋结构和高形状稳定性.
  • 观察到有效的结合于A和E环素.
  • 模仿者表现出显著的细胞吸收.
  • 在血清中实现了对蛋白质分解的完全耐药性,优于碳化合物级对照剂.

更多相关视频

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.4K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K

相关实验视频

Last Updated: Jun 29, 2025

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.9K
Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction
12:38

Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

Published on: August 9, 2011

17.4K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K

结论:

  • 双顶模体是设计螺旋接口探针的新和可通用的平台.
  • 这种方法克服了以前方法的局限性,提供了增强的稳定性和细胞透性.
  • 开发的探测器显示了针对蛋白质相互作用的治疗应用的前景.