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表位-MIPs和全蛋白MIPs的特定特征,如SARS-CoV-2的AFP和RBD所示

Xiaorong Zhang¹, Aysu Yarman², Norbert Kovács³

¹Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht Str. 24-25, 14476, Potsdam, Germany. xiaorong.zhang.1@uni-potsdam.de.

Mikrochimica acta

|April 4, 2024

概括

与整个蛋白质打印的聚合物相比,Epitope打印的聚合物对检测alpha-fetoprotein和SARS-CoV-2尖端蛋白的非特异性结合性降低,成本更低.

科学领域:

生物材料科学生物材料科学
分析化学分析化学
分子生物学分子生物学

背景情况:

分子印记聚合物 (MIP) 是具有定制识别特性的合成受体.
精确检测像α-fetoprotein (AFP) 和SARS-CoV-2尖端蛋白受体结合域 (RBD) 这样的生物标志物对于诊断至关重要.

研究的目的:

开发和比较表皮质MIP和蛋白质MIP用于AFP和RBD检测.
评估开发的MIPs的绑定亲和力,特异性和非特异性绑定特征.

主要方法:

通过使用体或整个蛋白质模板的 scopoletin 的电聚合制备 MIP 纳米膜.
使用导电原子力显微镜进行表征.
通过K_D值和非特异性结合测量来评估亲和力和交叉反应性.

主要成果:

与蛋白质MIP相比,Epitope-MIP表现出较高的印记腔的密度.
无论是表位MIP还是蛋白质MIP,它们对各自的目标 (AFP和RBD) 都显示出可比较低的纳米分子亲和力.
与蛋白质-MIPs相比,Epitope-MIPs显著减少了与人血清白蛋白 (HSA) 的非特异性结合.

结论:

在蛋白质识别方面,以皮印制的聚合物具有优势,因为其减少了非特异性结合,并可能降低了生产成本.
印记腔的尺寸会影响非特异性结合,这使得表皮-MIP成为敏感和特异性蛋白质检测的有希望的替代品.

关键词:

阿尔法-胎儿蛋白 (AFP) 是一种有分子印记的聚合物.在SARS-CoV-2的受体结合域 (RBD) 中.

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