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Updated: Jun 29, 2025

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
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在固定小鼠海马神经元的突触中检测酸

Svenja Bolz1, Natalie Kaempf2, Maria Muehlbauer2

  • 1Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany; Faculty of Biology, Chemistry, Pharmacy, Freie Universität Berlin, 14195 Berlin, Germany.

STAR protocols
|April 4, 2024
PubMed
概括

这项研究引入了一种新的方法,用于可视化突触中的酸丁酸4,5-双酸盐 (PI(4,5) P2的变化. 这种技术有助于理解PI{4,5) P2

关键词:
抗体是对抗体的一种.细胞生物学 细胞生物学细胞膜的细胞膜.显微镜的使用方法神经科学是一个神经科学.蛋白质的表达和净化过程

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Phosphoinositides and PIPs01:42

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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