转录因子PDX1对于器官分化至关重要,它调节了SERCA-依赖的Ca2+感觉神经元中的稳态
Jami L Saloman1, Ariel Y Epouhe2, Catherine F Ruff2
1Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Department of Neurobiology, Center for Neuroscience and Center for Pain Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Cell calcium
|April 4, 2024
概括
胰腺和十二指肠本体箱1 (PDX1) 是新发现的在感觉神经中,调节处理. 神经损伤后PDX1水平和功能下降,影响疼痛感知.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 胰腺和十二指肠本体箱1 (PDX1) 是胰腺发育的关键转录因子.
- 以前的研究表明PDX1的表达仅限于胃肠道.
- PDX1在神经系统中的作用在很大程度上仍未被探索.
研究的目的:
- 为了研究PDX1在感官神经元中的存在和功能.
- 确定PDX1在 afferents中 (Ca2+) 处理中的作用.
- 探索PDX1在神经病伤害反应中的参与.
主要方法:
- 利用一种依赖于基因的记者系统,在体内跟踪PDX1的表达.
- 采用Fura-2成像来评估传感神经元中的Ca2+动态.
- 在轨下神经损伤后检查PDX1和SERCA2B表达的变化.
主要成果:
- 鉴定PDX1表达在与毛囊相关的,向皮肤投射的感觉神经的亚群中.
- 在原始感官神经元中,PDX1调节了sarco/endoplasmic网膜运输ATPase 2B (SERCA2B) 异型的表达.
- 脑下神经损伤导致PDX1和SERCA2B的表达减少,并在PDX1表达的三角神经质神经元中失调Ca2+处理.
结论:
- PDX1在体感官系统表达,特别是在触觉 afferents.
- PDX1在感觉神经元中通过SERCA2B调节Ca2+处理中发挥作用.
- 主要 afferents 中的 PDX1 下调和 SERCA2B 失调可能会导致神经损伤后的疼痛.
相关概念视频
01:27Feedback Regulation of Calcium Concentration
3.4K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.4K
01:16Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
01:19Cadherins in Tissue Organization
3.0K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.0K