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机械压缩调节了瘤球状体入侵到3D原基质矩阵中的情况

Mrinal Pandey¹, Young Joon Suh¹, Minha Kim²

¹Department of Biological and Environmental Engineering, Cornell University, 306 Riley-Robb Hall, Ithaca, NY 14853, United States of America.

Physical biology

|April 4, 2024

概括

瘤压缩增加恶性细胞的运动和入侵,同时减少健康细胞的运动. 这突出了压缩压缩.

科学领域:

生物物理学的生物物理.
癌症生物学癌症生物学
细胞力学细胞力学

背景情况:

在狭窄的空间中瘤的生长会产生压力压力.
压缩在瘤入侵中的机械作用尚未得到充分研究.
张力对3D细胞外矩阵 (ECM) 的影响已知,但压缩的作用尚不清楚.

研究的目的:

为了研究恒定的压力负荷对3D原基质中的瘤球体的影响.
在压缩下分析单细胞动态和入侵行为.

主要方法:

经过修改的Transwell测定被用于应用恒定的压力负荷.
显微镜成像追踪了球体内的细胞动态和侵入ECM的情况.
研究了恶性 (MDA-MB-231) 和非瘤起源的 (MCF10A) 球体.

主要成果:

恶性乳腺瘤球状体在压缩下显示细胞运动率增加和ECM入侵.
非瘤源的上皮球体表现出细胞运动率下降,并且在压缩下没有明显的脱落.
在恶性和非瘤细胞之间观察到对压缩的不同反应.

结论:

压缩在健康和恶性上皮组织中起着不同的作用.
瘤机制,特别是压缩,是癌症入侵的关键因素.
了解机械力对于理解瘤的行为和进展至关重要.

关键词:

3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 3D ECM 这是入侵的入侵.瘤压缩瘤的压缩.瘤微环境是一个微环境.瘤球形状的球形状

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