在小鼠周植入期间定义一个以TFAP2C为中心的转录因子网络
Rui Gao1, Guang Yang2, Mengting Wang1
1Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China; Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
Developmental cell
|April 4, 2024
概括
这项研究揭示了转录因子 (TF) 如何调节早期胚胎发育. 一个以TFAP2C为中心的网络连接母亲的新陈代谢,表观遗传学和遗传学,以控制细胞两极分化.
科学领域:
- 发展生物学 发展生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 基因组学就是基因组学.
背景情况:
- 转录因子 (TFs) 对胚胎发育至关重要,但它们的调控机制,信号通路,基因组定位和对细胞命运的影响仍然不清楚.
- 了解这些因素对于阐明早期发育过程至关重要.
研究的目的:
- 在胚胎发育早期,研究以TFAP2C为中心的监管网络.
- 了解TFAP2C如何通过促进剂-增强剂相互作用调节细胞极化,并调节其他关键因素,如TEAD4和KLF5.5.
- 探索TF法规中母性因素,表观遗传修饰和遗传信息之间的联系.
主要方法:
- 使用CUT&RUN测序 (uliCUT&RUN-seq) 绘制TF全基因组定位的地图.
- 分析了以TFAP2C为中心的监管网络,包括促进者-增强剂相互作用.
- 研究了母亲的视网膜酸代谢和表观遗传修饰 (SINE脱甲基) 对TFAP2C.的作用.
- 研究了基因组印记和单核酸多态 (SNP) 对TF定位和基因表达的影响.
主要成果:
- 描述了一个以TFAP2C为中心的调节网络,涉及促进剂-增强剂相互作用,调节TEAD4和KLF5以调节细胞极化.
- 证明母亲的视网膜酸代谢通过SINEs的活性脱甲基化调节TFAP2C,将母体到zygotic过渡与细胞两极化联系起来.
- 表明基因组印记和SNP衍生的遗传信息影响TF定位和父母基因表达.
结论:
- 提出了一种三元模型,用于TF调节小鼠胚胎发育过程中的TFAP2C作为核心.
- 突出了在早期发育过程中,代谢,表观遗传和遗传信息在TF调节中的整合.
- 强调了控制细胞命运和胚胎模式的因素的复杂相互作用.
关键词:
根据TFAP2C的规定.胚胎发育过程中的胚胎发育.这是表观遗传学.印记是为了印记.代谢 代谢 代谢 代谢两极分化是一种极化.促进者-增强剂的相互作用.网红酸是什么意思 网红酸是什么意思单个核酸的多态性.转录因子的转录因子更多相关视频
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