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Updated: Jun 29, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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解读基质特异性揭示了CRISPR-Cas12a是一种具有内核和外核酶活动的双功能酶

Supreet Bhattacharya1, Ankit Agarwal1, Kalappa Muniyappa1

  • 1Department of Biochemistry, Indian Institute of Science, Bengaluru 560012, India.

Journal of molecular biology
|April 4, 2024
PubMed
概括

亚氨球菌 sp. 的情况. 卡斯12a (AsCas12a) 优先结合和降解分支DNA结构,如霍莱德结,独立于crRNA. 这揭示了新的基质特异性和外核酶活动,促进了对基因组编辑工具的理解.

关键词:
这就是Cas12a.霍利代交叉口的节假日的交叉口分支的DNA结构是分支的DNA结构.这就是为什么crRNARNA是 crRNA.基质特异性 基质特异性

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