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Rui Cao¹, Yihao Huang¹, Ruyu Li¹

¹Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction of the Ministry of Education & Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, College of Animal Science, Huazhong Agricultural University, Wuhan, China.

Environmental microbiology reports

|April 4, 2024

概括

微生物注射剂通过增强的保留和固定来提高堆肥质量. 这一过程增加了总含量,并减少了有害细菌和抗生素耐药性基因.

科学领域:

农业科学农业科学
环境微生物学环境微生物学
土壤科学土壤科学

背景情况:

堆肥肥料对于营养物质回收至关重要.
在堆肥中优化留和微生物动态对于肥料质量至关重要.
了解微生物注射剂对堆肥过程的影响至关重要.

研究的目的:

研究Bacillus和Saccharomycescerevisiae菌株对转化的影响.
为了分析微生物群落的变化,在肥堆肥时使用注射剂.
评估微生物注射对堆肥质量和病原体减少的影响.

主要方法:

用特定的微生物注射剂堆肥猪和肥.
监测堆肥温度,减少水分和细胞酶活性.
分析形式 (NH4+-N,NO3--N,TN) 和微生物社区结构 (16SrRNA测序).
量化关键的功能基因 (amoA, nxrA, nifH, nirK, nosZ, nirS) 和酶活动.
检测致病细菌和抗生素耐药性基因 (ARGs).

主要成果:

消毒剂增加了堆肥温度,减少了水分和细胞酶活性.
总含量增加了9%,刺激了NH4+-N和NO3--N的积累.
微生物群落发生了转变,Firmicutes减少,Actinobacteria和Bacteroidetes增加.
增加了amoA,nxrA和nifH基因拷贝数,以及nif/amo酶活动,同时减少了nirK,nosZ,nirS和nor.
特定的细菌 (Clostridium,Phascolarctobacterium,Eubacterium,Faecalibacterium) 与保留基因相关.
减少了致病性细菌和ARG (基诺,核酸,胺).

结论:

复合微生物接种剂在肥料堆肥过程中显著改善的保留和固定.
接种改变了微生物社区结构,并增强了关键的循环基因和酶.
微生物处理有效减少病原体和ARG,改善整体堆肥质量和安全性.

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