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在Fusarium mangiferae对紫外线辐射的动态反应

Shira Milo¹, Ritah Namawejje², Roi Krispin²

¹Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food and Environment. the Hebrew University of Jerusalem, Israel; Department of Natural and Life Sciences, The Open University of Israel, Israel.

|April 4, 2024

概括

像Fusarium mangiferae这样的叶状真菌没有对UVDNA修复的发育控制,与土壤真菌不同. 在康复过程中,紫外线暴露主要诱导与翻译相关的基因.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

适应多样化的环境依赖于DNA修复机制,特别是对于紫外线 (UV) 损伤.
以前的研究表明,在土壤传播的真菌Fusarium oxysporum中,紫外线修复的发育调节.
这项研究调查了叶子病原体Fusarium mangiferae的紫外线DNA损伤反应.

研究的目的:

确定Fusarium mangiferae是否表现出对紫外线修复,光解酶基因表达和紫外线诱导的突变发生的发育控制.
为了确定参与F. mangiferae在紫外线照射后恢复的基因和途径.

主要方法:

在F. mangiferae的不同发育阶段对紫外线生存,光活性依赖的修复和突变发生的比较分析.
在各种时间点进行F. mangiferae紫外线照射后的RNA测序 (RNAseq) 分析.
光聚酶的基因表达分析.

主要成果:

与F. oxysporum相比,F. mangiferae缺乏对光活化依赖的紫外线修复和光解酶基因表达的发育控制.
紫外线诱导的通过光激活减少突变发生在F. mangiferae的各个发育阶段都是一致的.
在F. mangiferae中,紫外线暴露显著诱导了与转化相关的基因,以与发育有关的方式.

结论:

紫外线DNA修复的发育调节在叶子和土壤传播的Fusarium物种之间有所不同.
F. mangiferae从紫外线损伤中恢复需要一个复杂的,时间依赖的反应,影响翻译,细胞循环和脂质代谢基因.

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