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与甲素固定相比,cytoLyt固定阻碍了胰岛素瘤相关蛋白1 (INSM1) 免疫活性

Michelle Garlin-Politis¹, Swikrity Upadhyay Baskota², Sarah Picon¹

¹Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York Presbyterian Hospital, New York, New York.

Journal of the American Society of Cytopathology

|April 4, 2024

概括

与甲素固定标本相比,在CytoLyt固定细胞块中,胰岛素瘤相关蛋白1 (INSM1) 染色显著减少,这可能导致神经内分泌瘤诊断中的假阴性.

科学领域:

病理学病理学病理学
在瘤学瘤学.
免疫组织化学免疫组织化学

背景情况:

胰岛素瘤相关蛋白1 (INSM1) 是神经内分泌瘤/癌瘤 (NETs/NECs) 的一个关键免疫组织化学标记物.
之前的研究表明,INSM1在CytoLyt固定细胞块和甲素固定组织中表现出类似的染色.
本研究研究了这两种固定方法之间的INSM1表达变异性.

研究的目的:

为了比较来自NET/NEC病例的CytoLyt固定和甲素固定细胞块中的INSM1免疫活性.
为了确定影响固定方法之间INSM1染色的潜在差异的因素.

主要方法:

追溯分析了20个NET/NEC病例,其中甲和CytoLyt都固定了细胞块.
在两种固定类型中评估INSM1染色 (正,负,模两可).
用百分比和强度对模两可的染色进行详细的评分.

主要成果:

所有的甲固定细胞块都显示出广泛的INSM1阳性 (>90%).
在65%的病例中,用CytoLyt固定的细胞显示出负的INSM1染色.
30%的CytoLyt固定样本显示出模两可的INSM1阳性.

结论:

与甲素固定标本相比,在CytoLyt固定的细胞块中,INSM1免疫组织化学染色显著减少.
在CytoLyt固定材料中减少INSM1染色会导致假阴性结果.
这一发现凸显了在使用CytoLyt固定细胞块时对NET/NEC的潜在诊断陷.

关键词:

这就是CytoLytyt.固定方式固定方式在INSM1中.神经内分泌的神经内分泌提高质量 - - 提高质量.

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