家禽土土壤修改对抗生素耐药性大肠杆菌的影响
Getahun E Agga1, Lisa M Durso2, Karamat R Sistani1
1USDA-ARS, Food Animal Environmental Research Unit, Bowling Green, Kentucky, USA.
Journal of environmental quality
|April 5, 2024
概括
与商业化肥相比,家禽垃圾 (PL) 肥料在玉米田中暂时增加了抗生素耐药的大肠杆菌 (AREc). 覆盖作物有助于减少耐药细菌,并在70天内恢复到基线水平.
科学领域:
- 农业科学 农业科学
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
背景情况:
- 矿物肥是昂贵的,不可再生的,这引发了人们对便替代品的兴趣,例如家禽垃圾 (PL).
- 肥料修正案将营养素和微生物,包括抗生素耐药的大肠杆菌 (AREc) 引入农业土壤.
- 了解AREc在土壤生态系统中的命运对于可持续农业和公共卫生至关重要.
研究的目的:
- 评估家禽垃圾 (PL) 土壤修改对玉米田抗生素耐药大肠杆菌 (AREc) 水平的影响.
- 为了比较PL,商业化肥 (CF) 和未经处理的对照 (UC) 对AREc流行的影响.
- 评估冬季作物系统 (休耕与覆盖作物) 对AREc动态的影响.
主要方法:
- 2017年5月至10月,在玉米田中进行了一项随机现场实验.
- 处理包括休与覆盖作物系统以及UC,PL或CF的春季应用.
- 在多个时间点分析了土壤样本,检测通用大肠杆菌,抗四环素 (TET ^ r) 大肠杆菌,第三代耐素 (3GC ^ r) 大肠杆菌和产生ESBL的肠杆菌.
主要成果:
- 与UC和CF相比,PL修改在第7天和第28天显著增加了通用大肠杆菌,TET^r大肠杆菌和3GC^r大肠杆菌.
- 在第70天,抗生素耐药的大肠杆菌水平恢复到基线,在第28天之后,肥料治疗之间没有显著差异.
- 覆盖作物在28日显著降低了TET^r E. coli,但没有影响3GC^r E. coli或ESBL产生肠道细菌的流行率.
结论:
- 禽垃圾土壤修改导致玉米田中抗生素耐药性大肠杆菌的暂时增加.
- PL对AREc的影响是暂时的,大约在70天内恢复到基线水平.
- 覆盖作物可以减轻农业土壤中某些抗生素耐药细菌的扩散.
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