RF-3192C和其他来自海洋内生植物Aspergillus niger ASSB4的多基类:结构分配和生物活性调查
Manar M Mahmoud1,2, Ahmed S Abdel-Razek2,3, Abdelaaty Hamed2,4
1Pharmacognosy Department, Faculty of Pharmacy, Helwan University, Helwan, Cairo 11795, Egypt.
概括
海洋真菌Aspergillus niger产生了新型化合物,包括RF-3192C,显示出对Pseudomonas aeruginosa和Bacillus subtilis的强烈抗菌活性. 还评估了对各种癌症细胞系的细胞毒性.
科学领域:
- 海洋天然产品化学 海洋天然产品化学
- 微生物的二次代谢产物
- 菌内植物研究研究
背景情况:
- 内生真菌,如Aspergillus niger,居住在像Laurencia obtusa这样的海洋生物中,是生物活性化合物的丰富来源.
- 由于其独特的化学结构和生物活性,海洋衍生天然产品在药物发现中显示出显著的潜力.
研究的目的:
- 从 Laurencia obtusa 获得的内性真菌 Aspergillus niger SB4 的二次代谢物分离和描述.
- 评估分离化合物的抗微生物和细胞毒性活动.
- 阐明新型化合物的化学结构和修改现有的化学结构.
主要方法:
- 菌生物质的甲醇提取.
- 使用色谱技术分离和净化化合物.
- 使用光谱方法 (1D/2D NMR,HRESIMS) 阐明结构.
- 抗菌活性测定对抗细菌和酵母.
- 在体外细胞毒性测定对人类癌细胞系 (HEPG2,HELA,A549,PC3,MCF7) 的一个小组进行了测试.
主要成果:
- 六种化合物被分离出来:RF-3192C (1),奥兰丁 (2),西因B (3),TMC-256A1 (4),环环--阿拉 (5) 和大脑酸A (6).
- 鉴定了五环多基化物RF-3192C (1),并首次阐明了其结构.
- 奥兰丁 (2) 的NMR赋值进行了修改.
- RF-3192C (1) 对 Pseudomonas aeruginosa 和 Bacillus subtilis 的抗菌活性较高,对酵母菌的活性中等.
- 所有分离的化合物都被评估为对多个癌症细胞系的体外细胞毒性活性.
结论:
- 内生真菌Aspergillus niger SB4产生了一系列具有重要的生物活动的二次代谢产物.
- RF-3192C (1) 是一种有前途的化合物,可用于抗菌药物开发.
- 这项研究有助于理解海洋源内植物的化学多样性及其在药物化学中的潜力.
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