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使用光谱技术探测6 MeV电子束辐射对血红蛋白的探测效应

Sarika Hinge¹, Sanjay Dhole¹, Arun Banpurkar¹

¹Department of Physics, Savitribai Phule Pune University, Pune, India.

Dose-response : a publication of International Hormesis Society

|April 5, 2024

概括

对人体血红蛋白A (HbA) 进行电子束辐射会改变其物理化学性质,降低界面张力并改变二次结构. 这些发现为生物细胞暴露于电子束辐射提供了指导方针.

科学领域:

生物物理学的生物物理.
材料科学材料科学材料科学
辐射化学辐射化学

背景情况:

人类血红蛋白A (HbA) 对于氧气运输至关重要.
了解辐射对生物分子的影响对于医疗和工业应用至关重要.
淋化HbA为辐射研究提供稳定性.

研究的目的:

研究6MeV电子束辐射对冷化HbA的物理化学性质的影响.
描述由不同电子束流动引起的结构和界面变化.
制定生物细胞暴露于电子束辐射的指南.

主要方法:

淋化HbA使用6 MeV电子束在5 × 10^14e-/cm^2和10 × 10^14e-/cm^2.2的波动下进行辐射.
描述涉及紫外线可见光谱学,富里埃变换红外光谱学 (FTIR) 和悬挂滴法用于界面张力分析.
使用FTIR胺带I解卷法进行了二次结构分析.

主要成果:

电子束辐射降低了HbA溶液的吸收强度,传导率和界面张力.
索雷特波段位置 (404 nm) 没有受到辐射波动的影响.
FTIR分析显示了HbA二次结构的显著变化:α-螺旋体含量下降 (8%至1%),而β-片 (19%至29%) 和β-螺旋体 (6.3%至15%) 的含量增加.
随着照射剂量的增加,界面张力从46.0mN/m降低到44.0mN/m.

结论:

6 MeV电子束辐射显著改变了冷化HbA的物理化学特性和二次结构.
观察到的界面张力下降和结构变化为辐射诱导的生物分子变化提供了洞察力.
这些发现为管理生物应用中的电子束辐射剂量提供了有价值的指导方针.

关键词:

里叶变换红外光谱学红外光谱学在紫外线下可见的紫外线.电子辐射辐射的电子辐射.血液中的血球蛋白.表面之间的张力.

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