相关实验视频
Updated: Jun 29, 2025
10:24
Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
10.7K
米OseIF6.1编码一个真核细胞翻译启动因子,对于谷物和其他物种的发展至关重要
Hongming Guo1,2, Jianqun Lv1,2, Xiangwen Su1,2
1Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, China.
Frontiers in plant science
|April 5, 2024
概括
米真核转化启动因子OseIF6.1对于谷物发育和生育是至关重要的. 它的调节影响谷物长度,花粉无菌性和种子设置,为植物生长提供了洞察力.
科学领域:
- 植物分子生物学 植物分子生物学
- 在大米中的基因功能
- 简介:真核转化启动因素
背景情况:
- 细胞转化启动因子6 (eIF6) 蛋白在细胞中至关重要.
- 虽然At-eIF6;1对阿拉比多普西斯胚胎发育至关重要,但它在米中的作用尚不清楚.
- 了解大米中的eIF6功能对于改善作物至关重要.
研究的目的:
- 调查OseIF6.1的功能,这是At-eIF6的同类;1.1.
- 阐明OseIF6.1在米生长,发育和繁殖中的作用.
- 确定参与其功能的OseIF6.1的交互伙伴.
主要方法:
- 在大米中OseIF6.1的基因淘汰和过度表达.
- 对谷粒长度,花粉活力和种子集的表型分析.
- 研究OseIF6.1与OsNMD3.3的相互作用.
- 通过敲击对OsNMD3功能进行分析.
主要成果:
- OseIF6.1 knockdown 降低了谷粒长度并导致花粉无菌;过度表达显示了相反的效果.
- OseIF6.1通过影响细胞扩张和增殖来调节米粒形状.
- OseIF6.1与OsNMD3相互作用,这是60S核糖体子单元的核出口因子.
- OsNMD3的淘汰导致生育能力降低,中的种子被放置.
结论:
- OseIF6.1在米粒发育和繁殖成功方面发挥着重要作用.
- OseIF6.1-OsNMD3相互作用对核糖体生物发生和植物生育非常重要.
- 这些发现提高了对eIF6在米开发中的作用的理解.
更多相关视频
08:39Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
1.6K
07:18Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
7.4K
相关概念视频
02:33Initiation of Translation
32.7K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
32.7K
01:47Transcription Initiation
16.4K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.4K
01:56Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
02:53Ribosomal RNA Synthesis
13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
02:28Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K