在腹腔镜抗逆流体手术前后食道粘膜的炎症反应
Pelin Ergun1,2, Sezgi Kipcak3,4, Nur Selvi Gunel4
1Department of Otolaryngology, Medical College of Wisconsin, Milwaukee, WI 53226, United States.
World journal of gastrointestinal surgery
|April 5, 2024
概括
腹腔镜反流体手术 (LARS) 显示食道中正在进行的炎症和恢复标志物. 细胞因子概况表明上皮质愈合,并且在手术后大约六个月内保持平衡.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 质子抑制剂是标准的,但不能治疗胃食道逆流症 (GERD).
- 腹腔镜反流体手术 (LARS) 为管理GERD症状和评估食道愈合提供了一个替代方案.
- 了解LARS后的炎症反应和上皮恢复对于开发改进的治疗方法至关重要.
研究的目的:
- 为了研究炎症生物分子在接受LARS的患者中的作用.
- 确定LARS后食道上皮的恢复时间表.
主要方法:
- 分析了22名LARS患者 (手术前和后) 和25名健康对照 (HC) 的食道活检.
- 采用了24小时的多通道内阻抗-pH监测和上胃肠内镜.
- 量化炎症分子使用逆转录聚合酶链反应和多重酶链接免疫吸收试验.
主要成果:
- 在LARS后的活检显示,与LARS前和HCs相比,促炎,抗炎和平静性细胞因子的增加.
- 特定的化学因子 (CCL17,CCL21) 和基因 (CCL2,表皮生长因子) 在LARS前高于HCs.
- 与LARS之前相比,AKT1,FGF2,HRAS和MAPK4的mRNA水平在LARS后下降.
结论:
- 在LARS后增加的前炎性细胞因子表明长期的上皮质炎症.
- 持续的恒常性细胞因子水平表明细胞平衡维持了大约六个月.
- 在LARS后观察到的抗炎反应表明抑制了炎症损伤和正在进行的组织恢复.
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