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Updated: Jun 29, 2025

PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays
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在盘中捕获内源性脂质,以及对蛋白质稳定性和活性的影响

Rupinder Singh Jandu1, Huaxu Yu2, Zhiyu Zhao1

  • 1Department of Biochemistry and Molecular Biology, Faculty of Medicine, Life Sciences Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

iScience
|April 5, 2024
PubMed
概括

了解蛋白质-脂质相互作用至关重要. 这项研究使用盘技术在特定的脂质环境中分离膜蛋白,揭示了它们的功能和稳定性的洞察力.

关键词:
膜架构是一种膜架构.蛋白质结构方面 蛋白质结构方面结构生物学是结构生物学.

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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

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相关概念视频

Lipids as Anchors01:32

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
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