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细菌原样蛋白的异位表达支持其在宿主-寄生虫共同进化的附着作用

Benjamin Huessy^1,2, Dirk Bumann², Dieter Ebert¹

¹Department of Environmental Sciences, Zoology, University of Basel, Basel 4051, Switzerland.

Royal Society open science

|April 5, 2024

概括

研究人员确定了一种类似Pasteuria原蛋白7 (Pcl7) 的蛋白质,该蛋白质调解了宿主-寄生虫的附着. 这一发现促进了对巴斯图里亚-达夫尼亚系统共同进化的遗传学的理解.

科学领域:

进化生物学进化生物学
遗传学遗传学是一个
微生物学微生物学

背景情况:

了解对抗性共同进化需要识别共同进化的基因.
细菌Pasteuria及其宿主Daphnia是共同进化的模型,但基因在很大程度上是未知的.
一项全基因组关联研究涉及Pasteuria原蛋白样蛋白7 (Pcl7) 在宿主寄生虫附着和共同进化的过程中.

研究的目的:

调查Pasteuria原蛋白样蛋白7 (Pcl7) 在宿主寄生虫共同进化的过程中调解附着的作用.
提供有关原样蛋白在达芬尼亚和帕斯图里亚共同进化的证据.

主要方法:

改造Bacillus thuringiensis以表达一种融合蛋白:来自Pasteuria ramosa的Pcl7 carboxy-terminus和一种B. thuringiensis的原蛋白样蛋白 (CLP).
测试了突变的Pcl7-Bt子与野生型Bt子对敏感和耐药的达夫尼亚基因型的附着.
评估宿主基因型特定的附着模式.

主要成果:

突变的Pcl7-Bt子,但不是野生型的Bt子,附着在像Pasteuria ramosa一样敏感宿主上的相同位置.
Pcl7-Bt子表现出基因型特定的附着性,易于与易受宿主基因型结合,但对抗性基因型的结合极小.
融合蛋白被正确表达和折叠,证实Pcl7的C端介导宿主特定的附着.

结论:

Pcl7的C末端对于以宿主基因型特定的方式调解Pasteuria与达芬尼亚的结合至关重要.
这项研究提供了强有力的证据,证明一种类似原蛋白的蛋白质在达芬尼亚和帕斯图里亚的共同进化中的作用.
这些发现为对宿主-寄生虫相互作用的遗传流行病学研究开辟了新的途径.

关键词:

细菌 thuringiensis 这种疾病.达夫尼亚大 (Daphnia magna) 是一个大的植物.帕斯图里亚拉莫萨 (Pasteuria ramosa) 是一个植物.像原蛋白一样的蛋白质.融合蛋白质是融合蛋白质的组成部分.主体寄生虫的相互作用

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