超声波增强的siRNA输送使用阴阳性脂质体-微泡复合体用于治疗状细胞癌
Bin Qin1, Xucai Chen1, Jianhui Zhu1
1Center for Ultrasound for Molecular Imaging and Therapeutics, University of Pittsburgh Medical Center, University of Pittsburgh, Pittsburgh, PA, USA.
Nanotheranostics
|April 5, 2024
概括
使用新型脂质体复合体的超声波向微泡化 (UTMC) 有效地将siRNA传递给癌细胞,在体内显著抑制瘤生长和EGFR表达. 这种非侵入性平台显示了针对性癌症治疗的前景.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 在瘤学瘤学.
背景情况:
- 微气泡 (MB) 对比剂与超声波向微气泡化 (UTMC) 结合,为特定地点的治疗性寡核酸递送提供了一个有希望的方法.
- 开发有效的治疗性寡核酸的传递系统,如siRNA,对于向的癌症治疗至关重要.
研究的目的:
- 通过使用一种新型的MB-脂质体复合体 (LPX) 来研究皮生长因子受体 (EGFR) 向状细胞癌 (SCC) 的siRNA的UTMC介导传递.
- 评估本平台在治疗癌症的治疗性siRNA的非侵入性,图像引导的向输送方面的有效性.
主要方法:
- 通过将阴阳性脂质体与MB结合而构建LPX,并加载siRNA.
- 试验室研究涉及培养SCC-VII-Luc细胞并用Luc siRNA LPX + UTMC对它们进行治疗,随后进行光酶测定.
- 在体内研究中使用了SCC-VII瘤携带的小鼠,用EGFR siRNA LPX + UTMC治疗,瘤EGFR表达量通过西部斑点测量,瘤体积通过3D美国成像评估.
主要成果:
- 通过UTMC介导的Luc siRNA LPX的输送在SCC-VII-Luc细胞中在0.25MPa至0.9MPa的声压下在体外实现了显著的光酶淘汰.
- 在体内,EGFR siRNA LPX + UTMC可使瘤EGFR表达减少约30%,并在第9天显著抑制瘤生长 (与对照组相比,瘤体积减少约40%).
结论:
- 开发的LPX平台与UTMC结合,在体外成功地实现了siRNA向SCC细胞的功能性传递.
- EGFR siRNA LPX + UTMC在体内显示出显著的瘤生长抑制和EGFR表达抑制,突出显示出其用于非侵入性,图像导向癌症治疗的潜力.
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