数据的独立获取揭示了尿性的深度血清蛋白质组变化
Zhao Wen-Jing1,2, Tan Rui-Zhi1, He Si-Yuan3
1Research Center of Intergated Traditional Chinese and Western Medicine, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China.
Frontiers in physiology
|April 5, 2024
概括
尿性 (UP) 涉及改变的血液蛋白质,可能与脏疾病和炎症有关. 研究人员确定了特定的蛋白质变化,可以作为患者这种常见疾病的生物标志物.
科学领域:
- 在病学中发现蛋白质组学和生物标志物.
背景情况:
- 尿路性 (UP) 是尿路病患者常见的,但不太了解的症状.
- 在UP患者中识别蛋白质变化可能会揭示潜在的生物学途径和潜在的生物标志物.
研究的目的:
- 为了识别来自尿性患者的血清样本中的差异表达蛋白质 (DEPs).
- 探索潜在的蛋白质生物标志物与尿性的发病相关.
主要方法:
- 来自54名尿性患者的血清样本 (DKD-UP,HN-UP,GN-UP) 和对照组 (无的尿血,无的CKD) 用LC-MS/MS数据独立获取 (DIA) 进行分析.
- 7075个和959个蛋白质的量化.
- 与对照组相比,对UP子组中DEP的交叉分析.
主要成果:
- 与负面对照相比,在UP子组中确定了许多上调和下调的DEP.
- 在UP患者中与CKD组相比,在UP患者中发现了67种常见的DEP (40个上调,27个下调).
- 确定了与线粒体功能,炎症,损伤和神经功能相关的关键DEP.
结论:
- 这项研究提供了关于尿性的分子机制的见解.
- 已识别的DEP代表尿性的可信生物标志物,有助于诊断和管理.
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