尿液利波阿巴比诺南在患有唾液阴性肺结核的人群中
P Ajantha1, Man Mohan Puri1, Devika Tayal2
1Department of TB and Chest, National Institute of Tuberculosis & Respiratory Diseases, New Delhi, India.
The Indian journal of medical research
|April 5, 2024
概括
使用子纳米技术检测尿液利波阿巴比诺南 (LAM) 显示出在艾滋病毒阴性个体中诊断涂抹阴性肺结核 (TB) 的前景. >0.615 ng/ml的门值表明了结核病的微生物学确认.
科学领域:
- 传染性疾病 传染性疾病
- 纳米技术 纳米技术
- 生物标志物检测检测 生物标志物检测
背景情况:
- 肺结核 (PTB) 的唾液显微镜具有较低的灵敏度.
- 目前的指导方针建议核酸放大测试 (NAAT) 和培养涂抹阴性PTB.
- 侧流尿液利波亚比诺南 (LF-LAM) 通常用于艾滋病毒阳性个体的结核病诊断.
研究的目的:
- 评估尿路LAM检测使用子纳米技术在艾滋病病毒阴性,样阴性PTB个体.
- 为了确定尿路LAM的诊断值,而不依赖于艾滋病毒状况.
主要方法:
- 基于子的纳米技术ELISA技术被用于量化尿液LAM.
- 参与者包括50名涂抹阴性PTB,15名涂抹阳性PTB和15名TB后续患者.
- 用ROC曲线分析将尿液LAM水平与唾液涂抹,NAAT和培养结果进行了比较.
主要成果:
- 在培养阳性PTB中,尿液LAM的平均值为0.84 ng/ml,在培养阴性涂抹阴性PTB中为0.49 ng/ml.
- 对于通过NAAT确认的涂抹阴性PTB,尿液LAM的平均值为0.76 ng/ml.
- 尿路LAM的最佳切断值为0.695 ng/ml (NAAT) 和0.615 ng/ml (培养). 尿路LAM的最佳切断值为0.695 ng/ml (NAAT) 和0.615 ng/ml (培养).
结论:
- 通过子纳米技术量化的尿液LAM水平,可以帮助诊断HIV阴性个体的涂抹阴性PTB.
- 尿中LAM值>0.615 ng/ml强烈表明微生物学确认了结核病.
- 这种方法为PTB提供了潜在的诊断工具,特别是在资源有限的环境中.
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