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八胺通过维持Caco2细胞的基底自来减轻肠道屏障损伤

Xiaoli Liu¹, Yan Zhou², Yu Zhang³

¹School of Basic Medical Sciences, Binzhou Medical University, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264003, P.R. China.

Molecular medicine reports

|April 5, 2024

概括

八胺通过通过SSTR2维持细胞活性和自,保护肠道屏障功能,这对于预防炎症损伤至关重要.

科学领域:

胃肠病学胃肠病学
细胞生物学细胞生物学
分子医学是分子医学.

背景情况:

肠道粘膜屏障对内部环境的稳定性至关重要,并与肠道炎症有关.
八甲胺 (OCT) 在肠道损伤方面表现有前途,但其机制尚不清楚.

研究的目的:

使用体外炎症模型研究Octreotide对肠粘膜屏障功能的保护作用.
阐明 somatostatin受体2 (SSTR2) 在 Octreotide 作用机制中的作用.

主要方法:

脂聚糖 (LPS) 诱导的炎症细胞模型.
细胞计数套件-8测定活力,跨表皮电阻和FITC-dextran 4测定透性.
对SSTR2的基因沉默,免疫阻塞,RT-qPCR用于紧密结合蛋白 (zona occludens 1).
电子显微镜和LC3-II/LC3-I转换用于自评估.

主要成果:

治疗LPS减少了细胞活力,增加了肠道透性,并破坏了紧密的结节 (zona occludens 1).
自标志物 (LC3) 在LPS后显示出动态变化.
依赖SSTR2的Octreotide治疗恢复了细胞活性,降低了透性,并使紧结蛋白和自正常化.

结论:

通过SSTR2介导的奥克特雷奥提德,保护肠道屏障功能.
OCT维持肠道上皮细胞的基础自和细胞活性,抵消炎症诱导的功能障碍.

关键词:

的Caco2细胞.自自是自的过程.肠道屏障的功能肠道屏障的功能肠道上皮细胞肠道上皮细胞八重胺的使用方法索马托斯塔丁受体索马托斯塔丁受体

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