晚期内体pH的崩会通过V-ATPase和RILP引起快速的Rab7反应
Ryan J Mulligan1,2,3, Magdalena M Magaj4,2, Laura Digilio1
1Department of Cell Biology, University of Virginia, Charlottesville, VA 22908, USA.
Journal of cell science
|April 5, 2024
概括
双LLOMe中和了晚期内的pH值,破坏了蛋白质平衡和受体循环. 这揭示了涉及V-ATPase和RILP调节内体pH和Rab7激活的新途径.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 内体-溶解体贩运需要通过H+-V-ATPase进行内体酸化,以获得适当的溶解体pH.
- 破坏pH平衡会损害溶酶体功能和蛋白质平衡,影响蛋白质的合成和降解.
- lysosomotropic 化合物可以中和内体的pH值,影响细胞过程.
研究的目的:
- 调查二胺LLOMe对晚期内分泌体 (LEs) 和它们相关的贩运途径的影响.
- 阐明LLOMe影响LE pH和Rab7激活的分子机制.
- 识别调节内体 pH 和 Rab7 功能的新途径.
主要方法:
- 用LLOMe和化 (NH4Cl) 处理哺乳动物细胞以诱导pH中和.
- 对Rab7激活,内体管道和CI-M6PR贩运的分析.
- 研究V-ATPase V1G1亚单元的组装及其与Rab7和RILP的相互作用.
主要成果:
- 在没有 lysosomal 膜通透的情况下,LLOMe 中和 LE pH,导致 Rab7 的过活化.
- pH中和破坏了内体管道和CI-M6PR循环.
- 在内体膜上增加的V-ATPase V1G1亚单元组合通过RILP稳定了与GTP结合的Rab7.
结论:
- 提出了一种新的途径,其中V-ATPase和RILP合作调节LE pH和Rab7激活.
- 这一途径有助于在生理内体细胞成熟和饥饿期间的pH控制.
- 通过 lysosomotropic 化合物或疾病状态的病理性 pH 中和可能涉及这种机制.
关键词:
酸性化是指酸性化的过程.晚期内分泌体的末期内分泌体是什么lysosomal 细胞膜的通透性.膜贩运活动 膜贩运在RILP中,RILP是指RILP.拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉布拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉拉这是一个V-ATPase.相关概念视频
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