Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

[Health risk assessment of <i>Pseudomonas aeruginosa</i> in source water of the Yangtze River Basin in China].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2026
Same author

A high-temperature superconducting compact Shanghai-Lanzhou electron beam ion trap for precision measurement of highly charged ions.

The Review of scientific instruments·2026
Same author

[Mechanism of actions and reflections of Masquelet induced membrane technique in diabetic wound].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2026
Same author

Isotope Abundance Measurement by Molecular Coulomb Explosion: Proof of Concept and Initial Performance Evaluation for Carbon and Oxygen Isotope Abundance.

Journal of the American Society for Mass Spectrometry·2026
Same author

[Classification of neurological recovery trajectories in patients with spontaneous intracerebral hemorrhage and construction of a meteorological factor-based predictive model for neurological function outcomes].

Zhonghua yi xue za zhi·2026
Same author

[Efficacy and reproducibility of a novel robotic-assisted system for anterior cruciate ligament reconstruction in <i>in vitro</i> bone tunnel drilling].

Zhonghua yi xue za zhi·2026

相关实验视频

Updated: Jun 29, 2025

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K

一个高分辨率的反应显微镜,具有通用双区域时间聚焦方法.

Y Gao1,2, T Cao1,2, K Z Lin1,3

  • 1Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China.

The Review of scientific instruments
|April 5, 2024
PubMed
概括

一个带有新型飞行时间谱仪的新反应显微镜改善了离子-原子碰撞研究. 这种创新的设计通过优化聚焦时间和减少用于反弹离子检测的噪音来提高精度.

更多相关视频

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
14:26

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

13.3K

相关实验视频

Last Updated: Jun 29, 2025

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.9K
In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy
14:26

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

Published on: January 19, 2011

13.3K

科学领域:

  • 原子和分子物理 原子和分子物理
  • 化学物理 化学物理
  • 实验物理实验物理学

背景情况:

  • 离子-原子碰撞是物理学和化学的基本过程.
  • 精确测量反弹离子动量对于理解碰撞动态至关重要.
  • 现有的光谱仪面临着噪音和聚焦限制的挑战.

研究的目的:

  • 介绍一种用于离子-原子碰撞研究的新型反应显微镜.
  • 引入具有增强聚焦能力的创新飞行时光谱仪.
  • 为了证明反弹离子运动量测量的精度提高.

主要方法:

  • 开发一种反应显微镜,配备一个双加速区域飞行时间光谱仪.
  • 对充电碎片实施飞行时间聚焦方法.
  • 将光谱仪轴倾斜12°以避免网格障碍并抑制噪声.
  • 使用62.5 keV/u He2+离子和原子束测试系统.

主要成果:

  • 为具有不同速度的碎片实现了最佳时间聚焦条件.
  • 成功地抑制了离子束轨迹中的残留气体噪声.
  • 在测试实验中获得了0.068原子单位的反弹纵向动量分辨率.
  • 在精确的离子-原子碰撞研究中证明了新型配置的有效性.

结论:

  • 新型反应显微镜和飞行时间谱仪为离子-原子碰撞研究提供了卓越的精度.
  • 灵活的聚焦方法和倾斜的几何形状解决了以前设计的关键局限性.
  • 这一进步有助于对碰撞动态进行更详细的研究.