用Candida tropicalis的代谢工程来高效地生产1,2,4-butanetriol
Jingyun Li1, Yuanyuan Xia1, Bo Wei1
1Key Laboratory of Industrial Biotechnology, Ministry of Education, & School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Biochemical and biophysical research communications
|April 5, 2024
概括
工程酵母通过优化铁代谢和NADPH再生来增强1,2,4-butanetriol的生产. 这种微生物过程有效地将玉米水解盐转化为有价值的化学前体.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 1,2,4-butanetriol是制药和能量材料的关键前体.
- 现有的生产方法在效率和基质利用方面面临挑战.
- 工程微生物菌株为化学合成提供了一个可持续的替代方案.
研究的目的:
- 为了提高1,2,4-butanetriol的生产效率,使用一种工程化Candida tropicalis菌株.
- 为了克服诸如酸盐积累和NADPH缺乏症等局限性.
- 探索使用玉米水解剂作为一个具有成本效益的基材.
主要方法:
- 在转录水平上调节铁代谢,以增强西隆酸脱水酶活性.
- 在NADPH再生途径中过度表达基因以解决辅因子失衡.
- 优化发酵条件,包括用碳酸控制pH值.
- 使用玉米水解剂作为发酵原料.
主要成果:
- 增强的细胞内铁离子可用性增加了二甲酸脱水酶活性2.2倍.
- 优化的NADPH再生导致1,2,4-butanetriol产量为3.2 g/L.
- 用碳酸控制pH值进一步提高了产量,达到4g/L (111%的改善).
- 使用玉米水解剂实现了1.2,4-butanetriol的3.42g/L的生产.
结论:
- 铁代谢的转录调节和战略基因过度表达是改善1,2,4-butanetriol生产的有效策略.
- 经过工程改造的Candida tropicalis菌株显示出具有重要的潜力,可以将纤维素生物质转化为有价值的化学物质.
- 这项研究建立了一个新的微生物平台,用于从玉米水解盐中可持续生产1,2,4-butanetriol.
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