阿拉比多普西斯的免疫核心受体BAK1和Pseudomonas注射器效应器HopF2之间的关联机制
Rui Han1, Tongtong Zhu1, Lili Zhou1
1State Key Laboratory of Maize Bio-breeding, Ministry of Agriculture Key Laboratory for Crop Pest Monitoring and Green Control, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Biochemical and biophysical research communications
|April 5, 2024
概括
植物免疫受体Brassinosteroid激活激酶1 (BAK1) 与细菌效应物HopF2.2相互作用. 这项研究揭示了参与其协会的具体地区,有助于制定对植物病原体的战略.
科学领域:
- 植物免疫学 植物免疫学
- 分子植物病原体相互作用
- 生物化学 生物化学
背景情况:
- 氨酸激活激酶1 (BAK1) 是一种重要的植物免疫核心受体.
- 来自Pseudomonas syringae的细菌效应剂HopF2通过与BAK1.1相互作用来抑制阿拉比多普西斯的免疫力.
- 目前尚不清楚HopF2-BAK1相互作用的确切机制.
研究的目的:
- 阐明阿拉比多普西斯BAK1和Pseudomonas注射器HopF2.2之间相互作用的分子机制.
- 确定BAK1和HopF2.2之间的关联所涉及的特定领域和地区.
- 为开发用于管理细菌性植物疾病的新策略提供基础.
主要方法:
- /交换质谱法 (HDX-MS) 用于绘制交互界面的地图.
- 位点定向突变发生,以验证关键相互作用残留物.
- 蛋白质-蛋白质对接模拟 (ClusPro) 和激酶活性测试.
主要成果:
- BAK1 (BAK1-KD) 的激酶域足以进行HopF2结合.
- 在BAK1-KDN叶和HopF2头子域中的特定区域对于它们的分子间相互作用至关重要.
- 突变分析和对接支持识别的交互接口.
结论:
- 这项研究阐明了植物免疫受体BAK1和细菌效应剂HopF2.2之间的相互作用机制.
- 了解这种相互作用是开发阻断HopF2功能的新杀菌剂的关键.
- 这些发现为增强植物抗病能力提供了潜在的途径.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
cAMP-dependent Protein Kinase Pathways
6.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.3K
MAPK Signaling Cascades
5.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.5K
Interactions Between Signaling Pathways
6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Assembly of Signaling Complexes
5.8K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.8K
Bacterial Signaling
32.1K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.1K


