西申万通过调节内分泌网膜压力来增强肠道屏障功能,以改善患有腹刺激性肠综合征的小鼠
Yucui Zhao1, Jiaguo Zhan1, Congying Sun1
1Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, China; School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
概括
西申万 (SSW) 通过增强自和抑制ER压力,改善了腹性刺激性肠综合征 (IBS-D) 小鼠的肠道屏障功能. 这种治疗也调节肠道微生物群,为IBS-D提供治疗方法.
科学领域:
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 腹性刺激性肠综合征 (IBS-D) 呈现为腹和腹痛,源于各种原因.
- 西申万 (SSW) 显示出IBS-D治疗的前景,但其机制尚未完全理解.
研究的目的:
- 在腹性易怒肠综合征 (IBS-D) 的小鼠模型中阐明Sishen Wan (SSW) 的治疗机制.
- 研究SSW对肠道屏障功能,肠道微生物群和相关分子通路的影响.
主要方法:
- 使用高乳糖饮食和慢性压力建立了IBS-D小鼠模型.
- 评估了SSW对内脏敏感性,排便,肠道过渡和肠道屏障完整性的影响 (MUC2,Occludin,紧密结节).
- 通过16S rRNA测序评估了结肠自,内分泌网膜 (ER) 压力标志物和肠道微生物组成.
主要成果:
- SSW治疗显著降低了内脏敏感性,改善了排便,并降低了肠道过渡速度和透性.
- 通过促进自和抑制ER压力,SSW增强了结肠粘液分泌.
- 施用SSW导致肠道微生物组的改变,增加了诸如Blautia和Muribaculum这样的有益细菌.
结论:
- 通过调节自和ER应激通路,SSW可以改善IBS-D患者的肠道屏障功能.
- 这些机制有助于SSW在IBS-D的治疗疗效.
- SSW还重塑了肠道微生物群,表明了多方面的治疗作用.
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