炎症诱导的林在功能障碍的内皮中介导酸盐排放,影响NO的生物可用性
Shamima Akhtar1, Komal Sagar1, Aishwarya Singh1
1Department of Biochemistry, AIIMS, New Delhi, India.
Nitric oxide : biology and chemistry
|April 5, 2024
概括
酸盐运输物Sialin对氧化 (NO) 的生物可用性和内皮功能障碍产生负面影响. 阻断氨酸会增加NO水平,为动脉样硬化和癌症提供潜在的治疗点.
科学领域:
- 内皮细胞生物学 内皮细胞生物学
- 分子医学是分子医学.
- 心血管研究研究心血管研究
背景情况:
- 内皮功能障碍是动脉样硬化的一个关键因素,但调节氧化 (NO) 生物可用性的机制尚不清楚.
- 外源性酸盐治疗在改善内皮功能方面取得了有限的成功,这表明内源性因素至关重要.
- 酸盐运输物Sialin已成为组织酸盐和酸盐水平的潜在调节剂.
研究的目的:
- 在内皮功能障碍的背景下,研究林在氧化 (NO) 生物可用性中的作用.
- 探索内皮细胞中素,自和NO生产之间的关系.
- 确定素作为内皮功能障碍相关疾病的潜在治疗标.
主要方法:
- 人类静脉内皮细胞 (HUVEC) 用TNFα或AT-2进行刺激,有或没有自调节器.
- 氧化,亚酸盐和亚酸盐的含量在细胞上浮物和溶解物中得到量化.
- 评估了基因表达 (qRT-PCR),细胞亡 (Annexin V-PI),单细胞粘附以及素和LC3的免疫光.
主要成果:
- 在体外激活的内皮细胞 (ECs),动脉样硬化斑块和瘤新血管中,素的表达高.
- 氨酸调节酸盐离子流出,并通过mTOR通路通过自细胞的负面调节.
- 阻断素增加了NO的生物可用性,自,细胞存活率和eNOS的表达,同时减少了单细胞粘附. 鉴定出LGALS8是氨酸的直接相互作用体,影响自和亡.
结论:
- 西雅林在调节NO的生物可用性和内皮细胞功能方面发挥着重要作用.
- 调节素活性,可能通过自调节,可以改善内皮功能障碍.
- 西阿林代表了一种有前途的新型治疗动脉样硬化和癌症治疗标.
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