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来自SHED-MSC的外体调节TPH-1衍生M1巨细胞中的极化和应激氧化指数

Ali Fallah¹, Abasalt Hosseinzadeh Colagar¹, Ayyoob Khosravi²

¹Molecular and Cell Biology Department, Faculty of Basic Science, University of Mazandaran, Babolsar, 47416-95447, Iran.

Archives of biochemistry and biophysics

|April 5, 2024

概括

脱落牙 (SHED-MSC-EXOs) 中的介质干细胞衍生外体 (MSC-EXOs) 可以调节氧化应激并抑制M1巨细胞的两极分化. 这些发现表明SHED-MSC-EXO具有治疗M1巨诱导疾病的潜力.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

在炎症性疾病中,M1巨细胞起着至关重要的作用.
介质干细胞衍生的外体细胞 (MSC-EXOs) 正在研究治疗潜力.
抑制M1巨细胞是新疗法的目标.

研究的目的:

为了研究从人体脱皮叶状牙衍生的干细胞 (SHED-MSC-EXOs) 对M1巨细胞两极分化的治疗效果.
评估SHED-MSC-EXOs对亲氧化剂和抗氧化剂指数的影响.
通过SHED-MSC-EXOmiRNAs的in-silico分析来阐明潜在的分子机制.

主要方法:

从THP-1衍生出的M1巨被分离和特征的SHED-MSC-EXO处理.
流细胞计用于分析M1特异性标记物 (CD80,CD86).
测量了亲和抗氧化剂指数;使用miRWalk 3.0.0进行了in-silico miRNA分析.

主要成果:

SHED-MSC-EXOs治疗显著降低了M1巨细胞标记物 (CD80,CD81).
治疗导致了减少的亲氧化剂和增加的抗氧化剂指数 (P <0.05).
在化分析中发现了8种SHED-MSC-EXO微RNA,能够准M1极化,线粒体和LPS诱导的氧化应激通路.

结论:

SHED-MSC-EXOs有效抑制M1巨细胞的两极分化,并调节氧化应激.
这些外体体显示出作为M1巨相关疾病 (M1IDs) 的治疗剂的潜力.
由于SHED-MSC-EXOs能够透目标细胞,因此它们对临床应用具有前景.

关键词:

异构体是什么? 异构体是什么? 外构体是什么?由LPS引起的氧化应激.巨细胞的两极分化线粒体的氧化应激是线粒体的氧化应激.在 SHED-MSC-EXO miRNAs 中.

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