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Porphyromonas gingivalis 感染改变了细胞外囊泡中的微RNA组成

Kayo Yoshida¹, Kaya Yoshida¹, Yasuhiro Mouri²

¹Department of Oral Healthcare Promotion, Tokushima University Graduate School of Biomedical Sciences, 3-18-15, Kuramoto, Tokushima, 770-8504, Japan.

Journal of oral biosciences

|April 5, 2024

概括

Porphyromonas gingivalis (Pg) 感染改变了细胞外囊泡 (EV) 的微RNA (miRNA) 概况,具有影响丰富的特定序列动机. 这表明先天免疫信号和miRNA特征在牙周炎中调节了EV通信.

科学领域:

口腔微生物学和免疫学
细胞外囊泡研究研究
微RNA生物学微RNA生物学

背景情况:

牙周炎通常是由 Porphyromonas gingivalis (Pg) 引起的,涉及口腔细胞相互作用的复杂变化.
细胞外囊泡 (EVs) 是牙周炎中细胞间通信的关键媒介.
在Pg感染细胞中的EVs的特定微RNA (miRNA) 含量尚未完全理解.

研究的目的:

识别和表征由Pg感染细胞 (Pg-EVs) 衍生的EVs丰富的miRNAs.
了解托尔类受体 (TLR) 信号在Pg-EVs中的miRNA丰富中的作用.
为了研究密集在Pg-EVs中的miRNA特征的序列动图.

主要方法:

在来自Pg感染TPH-1细胞的EV中的miRNA配置文件的微阵列分析.
在Pg-EVs中的miRNA水平与用脂多糖 (LPS) 刺激的EVs之间的比较.
评估TLR信号抑制对miRNA转录的影响,并调查miRNA序列动机.

主要成果:

与LPS刺激的EV相比,Pg-EV中显著增加了11个miRNA,包括miR-155.
Pg诱导的miR-155上调通过TLR2发生,但不是TLR4信号.
在Pg-EV中丰富的miRNA含有特定的序列基因,如AGAGGG和GRGGSGC,具有高的G+C含量.

结论:

Pg感染改变了EV miRNA的组成,独立于TLR4信号传输.
特定的序列动图,特别是那些高G+C含量的人,与Pg-EVs中的miRNA丰富有关.
天生的免疫受体信号传导和内在miRNA序列特征都可能在感染期间调节EV miRNA组成.

关键词:

细胞外囊泡细胞外囊泡巨细胞是一个巨细胞.牙周病是牙周病的一种疾病.葡萄牙牙牙病 Porphyromonas gingivalis 这种虫子是这是一个微型RNA.

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