相关实验视频

Updated: Jun 29, 2025

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

核酸诱导的ClpC寡合化及其与致病性Leptospira的ClpP异型的非偏好的关联

Surbhi Kumari¹, Arfan Ali², Manish Kumar¹

¹Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.

International journal of biological macromolecules

|April 5, 2024

概括

细菌的溶性蛋白酶-沙佩龙复合体,如莱普托斯皮拉查询器LinClpC和LinClpP1P2,降解了错误折叠的蛋白质. 它们的功能性蛋白酶活性需要形成异构复合体,并通过抗生素增强.

科学领域:

微生物学微生物学
蛋白质生物化学蛋白质生物化学
分子生物学分子生物学

背景情况:

细菌的类溶解蛋白酶-沙佩龙复合体对于消除错误折叠和聚合蛋白质至关重要.
勒普托斯皮拉采访者利用Clp-chaperones (ClpX,ClpA,ClpC) 可能与LinClpP1和LinClpP2异型相互作用.
LinClpC是一种具有两个ATPase域的I类护卫体,表现出核酸诱导的寡合化.

研究的目的:

为了研究莱普托斯皮拉查询ClpC (LinClpC) 和其相关的ClpP蛋白酶 (LinClpP1和LinClpP2) 之间的功能关联.
阐明LinClpC-LinClpP复合体内蛋白酶活性的结构和功能要求.
探索核酸和抗生素对蛋白质分解机制的影响.

主要方法:

尺寸排除色谱学尺寸排除色谱学
ANS染料结合试验的测定方法
动态光散射分析
使用β-casein和FITC-casein进行蛋白质分解活性测定.

主要成果:

ClpC经历了核酸诱导的寡合化.
ClpC与单独的ClpP1或ClpP2无偏好地结合在一起.
功能性蛋白质复合物只能由异质复合物LinClpP1P2与LinClpC结合而形成.
LinClpCP1P2复合体以能源独立的方式降解素,其活性由ATP/ATPγS和酸 (ADEP1) 增强.

结论:

异构三角体LinClpP1P2复合物,当与LinClpC伴侣组合在一起时,形成一个活跃的蛋白质分解机制.
在LinClpC的存在下,LinClpP1P2的结构重组对于蛋白酶活性至关重要.
像ADEP1这样的抗生素显著增强了LinClpP1P2复合物的蛋白酶活性.

关键词:

这是一种基化 (acyldepsipeptide).凯西诺分析蛋白质酶的作用在ClpC中,ATP酶是陪伴者.莱普托斯皮拉 (Leptospira) 是一个类动物.

更多相关视频

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance

Author Spotlight: Leptospira DNA Detection in Water for Environmental Analysis and Disease Surveillance

Published on: June 14, 2024

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Published on: July 1, 2011

相关概念视频

Ligand Binding and Linkage

Ligand Binding and Linkage

Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...

Translesion DNA Polymerases

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...