板素1和3的冷EM结构显示了板素异型之间的差异
Nazia Hussain1, Ashish Apotikar1, Shabareesh Pidathala1,2
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore, 560012, India.
Nature communications
|April 5, 2024
概括
泛素通道释放ATP. 这就是ATP. 研究人员确定了pannexin 3和两个pannexin 1变体的冷电子显微镜结构,揭示了关键的结构差异以及突变如何影响通道功能和ATP相互作用.
科学领域:
- 结构生物学是结构生物学.
- 分子生物物理学的分子生物物理学.
- 细胞生理学 细胞生理学
背景情况:
- 泛素是必不可少的单膜,大型毛孔通道,通过释放离子和ATP参与细胞通信.
- 三种pannexin异型 (PANX1,PANX2,PANX3) 呈现出不同的细胞功能,部分原因是它们的毛孔层残留物的变化.
研究的目的:
- 阐明板异形多样性和功能的结构基础.
- 研究特定残留物替代物对泛素道结构和ATP相互作用的影响.
主要方法:
- 使用冷电子显微镜 (cryo-EM) 确定了pannexin 3和两个pannexin 1变体的结构.
- 在潘尼克辛异型之间进行了结构比较,并分析了孔隙和前庭残留物替代的效应.
主要成果:
- 与pannexin 1和2相比,pannexin 3的冷-EM结构揭示了与pannexin 1和2相比,具有两个不同的腔室的更宽的前厅.
- 板素1的结构表明,在前厅中替换阴离子残留物减少了ATP相互作用.
- 一种pannexin 1生殖系突变 (R217H) 呈现出狭窄的孔隙,降低ATP相互作用,降低电压敏感性.
结论:
- 结构和功能分析突出显示了素异型之间显著的差异,特别是在它们的前庭和毛孔区域.
- 特定的突变可以全质地影响泛素通道关口和与ATP的相互作用,提供通道病变的洞察力.
更多相关视频
10:46Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
16.3K
09:25Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
Published on: January 9, 2015
46.2K
相关概念视频
01:28Cryo-electron Microscopy
3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
01:25Aquaporins
4.8K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
4.8K
01:25Structure of Cadherins
3.3K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.3K