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染色体规模的基因组测序和组装的Athetis lepigone

Alexander Yesaya¹, Lei Zhang¹, Chao Wu¹

¹Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, 518120, Shenzhen, China.

Scientific data

|April 5, 2024

概括

为新兴的昆虫害虫Athetis lepigone生成了染色体级的基因组组. 该资源有助于理解其遗传适应和害虫管理策略.

科学领域:

基因组学就是基因组学.
昆虫害虫生物学昆虫害虫生物学
分子昆虫学分子昆虫学

背景情况:

虫 (Athetis lepigone) 是一种重要的多虫害虫,在欧洲和亚洲造成作物损害.
有限的遗传资源阻碍了对A. lepigone适应机制的理解.

研究的目的:

为了呈现第一个染色体层次的基因组组装为Athetis lepigone.
为Athetis属提供一个基础的基因组资源.

主要方法:

使用PacBio的长读测序和Hi-C技术.
基因组组装大小为612.49Mb,使用20.9Mb的N50支架生成.
进行基因预测和功能注释,并对关键基因家族进行分析.

主要成果:

实现了612.49 Mb的高质量基因组组装,具有31个染色体大小的支架和37%的GC含量.
基因组完整性很高 (97.4%),有15965个功能性注释的基因.
确定了包括P450 (106),GST (37),UGT (27),COE (74) 在内的关键排毒基因家族.

结论:

A. lepigone的染色体级基因组是研究昆虫害虫进化和适应的宝贵资源.
这些基因组数据将促进对害虫管理和更广泛的Athetis属的研究.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.