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circRNA_8521通过海绵式miRNA-324来调节LC3A来促进塞内卡病毒A感染

Xiwang Yang¹, Rui Liu², Yunsha Du¹

¹Southwest University, College of Veterinary Medicine, Chongqing, 400715, China.

Veterinary research

|April 5, 2024

概括

新的研究揭示了循环RNAcirc_8521通过作为miR-324的海绵,增加LC3A表达,促进猪中的塞内卡病毒A (SVA) 感染. 这一发现为控制SVA爆发提供了潜在的新策略.

科学领域:

病毒学病毒学
分子生物学分子生物学
在RNA生物学,RNA生物学.

背景情况:

塞内卡病毒A (SVA) 由于囊泡性疾病的爆发,在猪业中造成重大经济损失.
目前的SVA控制方法不足,需要新的干预策略.
循环RNAs (circRNAs) 正在成为病毒感染中的关键调节剂,但它们在SVA病原发生中的作用尚未被探索.

研究的目的:

调查circRNAs在调节猪细胞中塞内卡病毒A (SVA) 感染中的作用.
为了确定特定的circRNA及其涉及SVA病变发生的分子机制.
探索SVA控制的潜在治疗点.

主要方法:

用RNA测序来分析SVA感染细胞中的circRNA表达特征,并控制PK-15细胞.
对circRNA-miRNA-mRNA相互作用的生物信息预测.
使用qRT-PCR,西部抹杀,双露西法酶记者测定和RNA拉下测定进行验证.

主要成果:

SVA感染显著改变了circRNA的表达,发现了67种差异表达的circRNA.
在被SVA感染的细胞中,Circ_8521显著上调,并被发现可促进SVA感染.
Circ_8521充当miR-324海绵,抑制miR-324与LC3AmRNA的相互作用,从而增加LC3A的表达并促进SVA感染.

结论:

通过调节circ_8521/miR-324/LC3A轴,Circ_8521在促进SVA感染方面发挥着至关重要的作用.
针对circ_8521介导的途径为SVA预防和控制提供了一个潜在的新战略.
这项研究阐明了一种新的分子机制,该机制是涉及circRNAs的SVA病原体的基础.

关键词:

在 LC3A 中.塞内卡病毒A型病毒环RNA 环RNA 是一个环RNA.这是一个小RNARNA.

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