相关实验视频

Updated: Jun 29, 2025

Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

CPK1-HSP90酸化和XopC2-HSP90效应因子的相互作用是木防御-病原体感染期间的对抗因素的基础

Yunxie Wei¹, Binbin Zhu¹, Ye Zhang¹

¹National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Key Laboratory of Biotechnology of Salt Tolerant Crops of Hainan Province, School of Tropical Agriculture and Forestry, Hainan University, Hainan Province, 572025, China.

The New phytologist

|April 6, 2024

概括

香细菌病 (CBB) 是一个主要的威胁. 热冲击蛋白90 (HSP90) 在麻豆-Xanthomonas phaseoli pv manihotis (Xam) 相互作用中起着双重作用,影响植物免疫力和病原体感染.

科学领域:

植物病理学植物病理学
分子生物学分子生物学
生物化学生物化学

背景情况:

香细菌病 (CBB) 是由Xanthomonas phaseoli pv manihotis (Xam) 引起的,严重影响了香的生产.
了解大麻-Xam相互作用的分子机制对于CBB管理至关重要.

研究的目的:

为了阐明热冲击蛋白90kDa (MeHSP90.9) 在麻和Xam之间的相互作用中的作用.
在宿主和病原体中识别与MeHSP90.9相互作用的蛋白质.

主要方法:

进行了蛋白质-蛋白质相互作用研究.
分析MeHSP90.9酸化及其对下游基因表达的影响.
研究了Xam蛋白XopC2对MeHSP90.9功能的影响.

主要成果:

MeHSP90.9表现出双重作用:通过MeCPK1的酸化促进植物免疫力,并在XopC2.2被抑制时促进Xam感染.
通过MeCPK1介导的MeHSP90.9的酸化增强了防御基因 (MeSRS1,MeWRKY20) 和免疫反应的转录.
XopC2 抑制了 MeHSP90.9 酸化,抑制了宿主免疫力,促进了病原体感染.

结论:

MeHSP90.9是麻-Xam相互作用的关键调节者,其酸化状态决定了其在防御或易感性方面的作用.
这些发现为基因改善麻病对CBB的耐药性提供了见解.

关键词:

在 HSP90 陪伴者.效应器效应器是一个有效的效应器.免疫反应的免疫反应.蛋白质相互作用蛋白质相互作用蛋白质的酸化是蛋白质的酸化.

更多相关视频

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Author Spotlight: Identifying Compensatory Pathways in Malaria Parasites Containing Hypomorphic Allele of Essential Protein Kinases

Published on: November 22, 2024

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

相关概念视频

Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

Protein Complex Assembly

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...