基于衍生和注射LC-MS/HRMS) 方法的完整的纳德罗隆II期氧代谢物的同时量化和鉴定
Panagiotis Sakellariou1, Polyxeni Kiousi1, Michael Petrou2
1Doping Control Laboratory of Athens, Institute of Biosciences and Applications, National Centre for Scientific Research "Demokritos", Athens, Greece.
使用液体染色学高分辨率质谱法 (LC-MS/(HRMS)) 和吉拉德试剂T (GRT) 的新方法准确量化尿液中的纳德罗隆代谢物. 这种经过验证的方法改善了反兴奋剂分析的检测和识别.
科学领域:
- * 分析化学 分析化学
- * 法医科学 法医科学
- * 生物化学 * 生物化学
背景情况:
- * 纳德罗隆是一种类固醇,在体育运动中被禁止使用.
- *检测尿液中的代谢物对抗兴奋剂工作至关重要.
- * 现有的方法可能对某些代谢物缺乏敏感性或特异性.
研究的目的:
- * 开发和验证一种敏感和选择性的方法来定量完整的纳德罗隆II期氧代谢物.
- *使用液体染色学-双重高分辨率质谱 (LC-MS/(HRMS)) 进行增强检测.
- *为了将新方法与传统的气体染色学-双重质谱法 (GC-MS/[MS]) 进行比较.
主要方法:
- * 用吉拉德试剂T (GRT) 来衍生尿样.
- * 在正电离模式下使用LC-MS/HRMS进行直接注射分析.
- * 葡萄糖和硫酸盐代谢物的染色分辨率和量化.
主要成果:
- * 该方法实现了纳德罗隆氧代谢物的敏感和选择性量化.
- *衍生促进了与类固醇骨干相关的强烈产物离子.
- * 纳德罗隆代谢物量化在1-100 ng/mL的范围内,具有结构识别.
- *方法验证遵循世界反兴奋剂机构 (WADA) 的标准.
结论:
- * 开发的LC-MS/HRMS) 方法与GRT衍生是有效的纳德罗隆代谢物分析.
- * 与传统的GC-MS/[MS]相比,这种方法提供了更好的检测和识别能力.
- *该方法适用于常规的反兴奋剂实验室和分泌研究.
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