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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

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在癌症中微卫星不稳定性检测:一种多重的qPCR方法,可以避免需要匹配正常样本.

Wei Chen¹, Yan Helen Yan¹, Blake Young¹

¹NuProbe USA, R&D and Innovation Department, Houston, TX, United States.

Clinical chemistry

|April 6, 2024

概括

VarTrace是一种新的定量PCR测定方法,可以在没有正常样本的情况下准确检测瘤DNA中的微卫星不稳定性 (MSI). 这种快速,低成本的方法提高了MSI测试的效率,特别是在低瘤百分比的情况下.

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Published on: April 11, 2016

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Last Updated: Jun 29, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Published on: April 11, 2016

科学领域:

分子生物学分子生物学
在瘤学瘤学.
遗传学是一个遗传学.

背景情况:

微卫星不稳定性 (MSI) 表明在结直肠癌等癌症中存在DNA不匹配修复缺陷.
目前的PCR-毛细管电泳 (CE) 方法需要正常样品和专用设备.
VarTrace是一种定量PCR (qPCR) 试验,旨在仅使用瘤DNA来评估MSI.

研究的目的:

开发和验证VarTrace,一种用于MSI检测的快速,低成本的qPCR试验.
消除在MSI测试中对正常DNA样本进行匹配的需要.
为了评估VarTrace的性能与标准PCR-CE测定对比.

主要方法:

用VarTrace测试了101个瘤样本,并与Promega OncoMate试验 (PCR-CE) 进行了比较.
使用人工样本和合成参考标准来评估检测极限.
评估了分析灵敏度,特异性和输入要求.

主要成果:

VarTrace成功分析了所有101个FFPE样本,具有100%的灵敏度和98%的特异性,与OncoMate相比.
该试验检测到高MSI (MSI-H) 的97%准确率降至10%的瘤.
分析研究显示,5%的变异性等位基因的检测频率极限和0.5 ng的输入极限.

结论:

VarTrace是一种快速,准确和经济的MSI检测试验,即使在瘤含量低的样本中也是如此.
测试不需要匹配正常DNA,简化了工作流程.
VarTrace提高了MSI分析的灵敏度,可能提高临床工作流程的效率和测试可访问性.