相关实验视频

Updated: May 6, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

通过海洋酵母 Wickerhamomyces subpelliculosus ZE75 的乙醇发酵的动态建模和优化

Heba Hawary¹, Abdel-Kareem M Marwa², Abdel-Hamied M Rasmey³

¹Botany and Microbiology Department, Faculty of Science, Suez University, Suez, 43221, Egypt. Heba.Hawary@sci.suezuni.edu.eg.

World journal of microbiology & biotechnology

|April 6, 2024

概括

海洋酵母 Wickerhamomyces subpelliculosus ZE75 通过使用海水增强了乙醇生产. 优化的发酵条件实现了高乙醇产量,表明了大规模的潜力.

科学领域:

微生物学微生物学
生物技术是生物技术.
生物能源是生物能源.

背景情况:

海洋环境拥有独特的微生物,具有潜在的工业应用.
维克拉摩菌 (Wickerhamomyces subpelliculosus) 是一种因其发酵能力而闻名的酵母物种.
高效的乙醇生产对于可持续的生物燃料开发至关重要.

研究的目的:

为了增强海洋酵母 Wickerhamomyces subpelliculosus ZE75.5.的乙醇生产.
分析动力参数并优化发酵条件以提高乙醇产量.
评估使用海水基媒介进行大规模乙醇生产的可行性.

主要方法:

从海洋沉积物中分离和识别海洋酵母 Wickerhamomyces subpelliculosus ZE75.
现型和基因型表征,包括内部转录间隔器 (ITS) 测序.
动力参数分析:最大特定乙醇生产率 (vmax) 和最大特定生长率 (μmax).
优化发酵参数:pH,盐度和葡萄糖度.
在100%天然海水基媒介中发酵.

主要成果:

Wickerhamomyces subpelliculosus ZE75表现出高乙醇生产能力,最初的产量为89.77gL-1.1.
最大特定乙醇生产率 (vmax) 是0.482g-1h-1在175gL-1的葡萄糖中.
最大增长特异性速率 (μmax) 在150gL-1葡萄糖下为0.141h-1.
在最佳的发酵条件下,以海水为基础的介质实现了0.5637g-1的乙醇产量.
在pH值为4.5的情况下,最大的乙醇产量达到104.04gL−1,特异性为0.1669gg−1h−1.1.

结论:

Wickerhamomyces subpelliculosus ZE75 是一个有希望的候选人,可以有效地生产乙醇.
基于海水的介质可以有效地用于酵母发酵,减少对淡水资源的依赖.
优化的发酵参数和确定的酵母菌株支持大规模工业乙醇生产的潜力.

关键词:

维克拉莫米塞斯 (Wickerhamomyces subpelliculosusus) 是一种状状的动物.生物燃料是一种生物燃料.乙醇发酵过程中的乙醇发酵.动力学研究动力学研究海洋酵母酵母是一种海洋酵母.优化优化优化优化

更多相关视频

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Light-Controlled Fermentations for Microbial Chemical and Protein Production

Published on: March 22, 2022

相关概念视频

Fermentation

Fermentation

Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

Bioreactor Controls-II

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

Designing Growth Media for Bioreactors

Designing Growth Media for Bioreactors

Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...

Biofuels

Biofuels

The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...