暴露会通过降低甲状腺激素受体/PGC1α信号来激活 mitophagy 在孕前
1Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, Anhui 230022, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract, Key Laboratory of Population Health Across Life Cycle, Anhui Province Key Laboratory of Reproductive Health and Genetics, Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Provincial Institute of Translational Medicin (Anhui Medical University), No. 81 Meishan Road, Hefei, Anhui 230032, China.
孕产妇暴露于会激活胎盘线粒,这是一种与孕前相关的过程. 这通过降低PGC1α表达发生,影响线粒体功能并增加可溶性Flt1 (sFlt1) 水平,从而导致先兆子的发展.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 孕期暴露是产前的危险因素.
- 孕前症的特征是被激活的胎盘髓.
- 关联,线粒和孕前的确切机制需要阐明.
研究的目的:
- 为了研究诱导的线粒活化激活的机制.
- 探索诱导的线粒和孕前之间的关联.
主要方法:
- 量化实时PCR,西斑,免疫光和免疫化学被用于分析预先怀孕胎胎盘和JEG3细胞中的线粒菌标记物.
- 为了研究分子通路,JEG3细胞被用化 (CdCl2),IOP酸 (IOP),3-甲基丁和PGC1α siRNA处理.
主要成果:
- 暴露增加了线粒体的标志物 (LC3BII/I,P62) 和降低了线粒体蛋白质 (TOM20,FUNDC1) 和PGC1α表达在胎盘组织和JEG3细胞.
- 减少PGC1α表达与增加可溶性Flt1 (sFlt1) 水平相关,这是与孕前相关的标志物.
- 3-甲基丁部分逆转了,IOP和PGC1αsiRNA对sFlt1表达的影响.
结论:
- 孕产妇暴露于会通过降低PGC1α表达的调节,可能通过甲状腺激素受体信号来触发胎盘髓.
- 这种由诱导的线粒途径与孕前的发病有关,部分是通过增加sFlt1水平.
- 向线粒或PGC1α可能为诱导的孕前提供治疗策略.
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