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识别基醇的葡萄化及其诱导在UDP-glucuronosyltransferases异型上
Haoqian Zhang1, Li Yang1, Dandan Shen1
1Department of Obstetrics and Gynecology, The third Affliated Hospital of Zhengzhou University, Zhengzhou, China; Zhengzhou Key Laboratory of Cervical Disease, The third Affliated Hospital of Zhengzhou University, Zhengzhou, China; National Clinical Research Center for Obstetrics and Gynecology, Henan Branch, The third Affliated Hospital of Zhengzhou University, Zhengzhou, China.
这项研究确定了关键的UDP-glucuronosyltransferases (UGTs),它们负责代谢环境污染物甲 (BPs). 结果揭示了参与BP排毒的特定UGT异型,以及对UGT表达的潜在影响. 关键词:基醇,UGTs,新陈代谢,排毒.
科学领域:
- 环境化学环境化学
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
背景情况:
- 甲 (BPs) 是广泛存在的环境污染物,具有工业应用.
- 了解BP代谢对于评估其环境影响和毒性至关重要.
研究的目的:
- 为了识别UDP-glucuronosyltransferase (UGT) 异型,参与甲醇的代谢消除.
- 调查BP代谢中的物种差异以及2,4,6-三二对UGT表达的影响.
主要方法:
- 用人类肝脏显微体 (HLM) 和尿素二酸葡萄糖酸 (UDPGA) 化二醇.
- 酶动力学分析 (迈凯利斯-门,基质抑制).
- 使用重组酶和抑制研究的UGT异型体的表型化.
- 使用来自猪,老鼠,子和狗的肝脏显微体来研究物种差异.
- 对HepG2细胞中UGT1A1和UGT2B7表达的2,4,6-tribromophenol影响的评估.
主要成果:
- 在HLM中形成了BP的单糖化物.
- 针对4-甲 (UGT1A6),2,4-二甲 (UGT1A1,UGT1A6,UGT1A8) 和2,4,6-三甲 (UGT1A1,UGT1A8,UGT2B4) 确定了特定的UGT异型.
- 暴露于2,4,6-三醇可能会诱导HepG2细胞中的UGT1A1和UGT2B7的表达.
结论:
- 在基醇的排毒中,UGT异构体起着重要的作用.
- 在BP代谢中存在特定物种的差异.
- 甲可能会影响某些UGT酶的表达,影响其自身的新陈代谢和其他异生菌的新陈代谢.
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