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Phase Contrast and Differential Interference Contrast Microscopy01:26

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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jun 29, 2025

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
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Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging

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使用带板显微镜进行反射成像.

Ranveig Flatabø1, Sabrina D Eder1, Thomas Reisinger1

  • 1Department of Physics and Technology, University of Bergen, Bergen, Norway.

Ultramicroscopy
|April 6, 2024
PubMed
概括
此摘要是机器生成的。

中性原子显微镜现在可以使用聚焦的束进行反射成像. 这种新的技术实现了微米以下的点尺寸,用于增强表面敏感性,非破坏性成像.

关键词:
中性显微镜的中性显微镜.这就是SHEM SHEM的意思.

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相关实验视频

Last Updated: Jun 29, 2025

Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
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Published on: November 25, 2009

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科学领域:

  • 表面科学是一门科学.
  • 原子物理 原子物理
  • 显微镜技术的使用方法

背景情况:

  • 中性原子显微镜提供非破坏性,表面敏感的成像.
  • 以前的方法使用了聚合光束 (针孔显微镜) 或聚焦光束进行传输.
  • 聚焦束反射成像以前没有被证明.

研究的目的:

  • 通过使用聚焦的束来演示反射成像.
  • 为了实现高分辨率成像的亚微米聚焦点大小.
  • 来自聚焦的束点的散射分布的特征.

主要方法:

  • 利用一个聚焦的束与一个原子光学元件 (区域板).
  • 通过在表面上扫描聚焦光束来获得反射图像.
  • 通过改变撞击光束角度来测量散射分布.

主要成果:

  • 通过聚焦束的反射图像获得的点尺寸降低到4.7μm ± 0.5μm.
  • 证明了将焦点能力降低到大约1μm的点大小.
  • 展示了从聚焦束点的散射分布的测量结果.

结论:

  • 在反射模式中成功实现了聚焦束显微镜.
  • 该技术为表面分析提供了高分辨率.
  • 开发了一种专门的探测器,用于增强显微镜实验.