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不同灭菌技术对测试化学品在土壤中的吸附和NER形成的影响
Rebecca Süßmuth1, Prasit Shrestha2, Cecilia Andrea Diaz Navarrete2
1Institute of Geology and Palaeontology - Applied Geology, University of Münster, Corrensstraße 24, 48149, Münster, Germany; Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Auf dem Aberg 1, 57392, Schmallenberg, Germany.
Chemosphere
|April 6, 2024
概括
自闭塞是生物降解和吸附研究中最有效的土壤灭菌方法,最大限度地减少对土壤特性和不可提取的残留物形成的影响. 亚酸是最不有效的,并且改变了土壤的pH值.
科学领域:
- 环境化学环境化学
- 土壤科学 土壤科学
- 微生物学 微生物学
背景情况:
- 关于土壤化学命运的经合组织标准测试依赖于无菌样本来评估非生物降解和解释非无菌结果.
- 确保绝育对土壤基质的影响最小化对于吸附和非可提取残留物 (NER) 研究数据的可比性至关重要.
研究的目的:
- 评估不同土壤灭菌技术的效率 (自封式,玛辐射,酸).
- 评估这些灭菌方法对测试化学品的土壤吸附和NER形成的影响.
主要方法:
- 实验遵循经合组织307 (生物降解) 和经合组织106 (吸收) 的指导方针.
- 在两种不同的土壤上应用了三种杀菌技术,使用14C标记的,氧尼尔和非标记的,阿特拉.
- 绝育效率通过盘子计数和RNA提取得到证实.
主要成果:
- 没有一种方法可以实现完全的土壤灭菌;自动化是最有效的.
- 酸处理导致无效的灭菌和土壤pH值的增加.
- 绝育方法影响了NER的形成,特别是自封和玛辐射.
- 试验化学品的吸附行为没有受到任何绝育技术的显著影响.
结论:
- 在土壤研究中,推自封式灭菌是最合适的灭菌技术.
- 必须仔细考虑杀菌方法,以避免生物降解和NER评估中的工件.
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