印醇-3-卡宾醇通过Nrf2/HO-1通路激活,改善了老雌性小鼠的卵巢损伤
Huiqing Hu1, Fangfang Li1, Fengyu Zhu2
1Department of Oncology, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, China; NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University), No 81 Meishan Road, Hefei 230032, Anhui, China.
Biochemical pharmacology
|April 6, 2024
概括
印醇-3-碳醇 (I3C) 通过减少纤维化和亡来保护卵巢老化. 这种化合物增强原始毛囊数量,并通过Nrf2和HO-1通路增强抗氧化防御.
科学领域:
- 生殖生物学 生殖生物学
- 老年学是一门学科.
- 分子毒理学分子毒理学
背景情况:
- 卵巢衰老是不孕不育和出生缺陷的重要原因之一.
- 氧化应激,亡和纤维化是导致卵巢衰老的关键因素.
- 确定缓解卵巢衰老的干预措施对于生殖健康至关重要.
研究的目的:
- 调查印-3-卡宾醇 (I3C) 对卵巢衰老的保护作用.
- 评估I3C对老化卵巢中氧化应激,亡和纤维化的影响.
- 阐明I3C在卵巢衰老中的作用背后的分子机制.
主要方法:
- 将I3C给年轻和老老的小鼠,然后进行组织学和生物化学分析.
- 免疫组织化学,Mason,Sirius红色和TUNEL染色用于纤维化和亡的评估.
- 西部涂抹,毛囊计数,雌性周期分析和体外细胞测试,以评估I3C的疗效和机制.
主要成果:
- I3C治疗抑制了卵巢纤维化和亡,并增加了原始卵泡数量.
- 在机械上,I3C促进了核因子-红素2相关因子 (Nrf2) 的核转移,并上调了血红氧酶1 (HO-1) 的表达.
- I3C增强了细胞活力,减少了氧化应激和细胞损伤的标志物,其效果取决于Nrf2/HO-1激活.
结论:
- 印醇-3-碳醇表明对抗卵巢衰老的保护作用.
- I3C通过对抗纤维化,亡和氧化应激减轻卵巢损伤.
- I3C可以作为延缓卵巢衰老和保持生育能力的潜在治疗剂.
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