相关实验视频
Updated: Jun 29, 2025
08:49
A 3D Organotypic Melanoma Spheroid Skin Model
Published on: May 18, 2018
15.7K
黑色素瘤远程转移的分子进化
Vladimir Bezrookove1, Sara Kianian1, Lea McGeever2
1Center for Melanoma Research and Treatment, California Pacific Medical Center, San Francisco, California, USA; California Pacific Medical Center Research Institute, San Francisco, California, USA.
The Journal of investigative dermatology
|April 6, 2024
概括
黑色素瘤转移的途径是复杂的. 基因组分析显示出明显的拷贝数变化,并表明远程传播可能会绕过淋巴结,影响黑色素瘤进展的向治疗.
科学领域:
- 在瘤学瘤学.
- 遗传学 是一个遗传学.
- 癌症生物学 癌症生物学
背景情况:
- 原发性黑色素瘤向转移性疾病的进展,尤其是淋巴结和远部位的进展,仍然不太清楚.
- 基因组的改变在黑色素瘤的演变和转移中起着至关重要的作用.
研究的目的:
- 研究从原发性瘤到转移性瘤的黑色素瘤进展过程中的基因组多样性和克隆模式.
- 了解转移过程中所涉及的途径,并识别潜在的驱动因素.
主要方法:
- 在匹配的原发性黑色素瘤和转移上使用光现场杂交 (FISH) 的比较基因组杂交.
- 下一代测序 (NGS) 分析变异性基因频率并确定遗传驱动因素.
- 分析了来自17名患有原发性黑色素瘤,淋巴结和远程转移的患者的样本.
主要成果:
- 在整个转移级联中,FISH分析发现了单调的拷贝数变化,包括PHIP增益和PTEN损失.
- 特定的副本数变化 (BPTF,MITF,NCOA3) 显示了淋巴结与远程转移中的差异性丰富.
- NGS揭示了远程转移中变异性等位基因频率的降低,以及淋巴结和远程转移之间的独特遗传驱动因素.
- 有证据表明,远程黑色素瘤转移并不总是源于淋巴结转移.
结论:
- 在黑色素瘤转移过程中,酸3-激酶和MITF通路可能会被丰富.
- 基因组分析提供了对黑色素瘤的克隆进化和转移能力的见解.
- 这些发现对开发向治疗和了解黑色素瘤传播有潜在的影响.
相关概念视频
02:30Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
02:07Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
02:21Cancers Originate from Somatic Mutations in a Single Cell
11.9K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
11.9K
01:30Skin Cancer
4.1K
Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...
4.1K