相关实验视频

Updated: Jun 29, 2025

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

缺氧作为组织形成的刺激:在微手术血管化的组织工程构造中,有机生成的概念是微手术血管化的组织

Ahmad Eweida¹, Elli Sandberg², Oliver Ritthaler²

¹Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center Ludwigshafen, University of Heidelberg, Ludwig-Guttmann-Str. 13, 67071, Ludwigshafen, Germany; Department of Head, Neck and Endocrine Surgery, Faculty of Medicine, University of Alexandria, Alkhartoum Square, 5372066, Alexandria, Egypt.

Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery

|April 6, 2024

概括

带有动脉静脉循环 (AVL) 的组织工程结构促进了放射治疗后的再生. 在AVL结构中的缺氧激活HIF1-SDF1轴,增强原生细胞招募和骨质分化,改善骨再生.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

轴向血管化对于再生大型,临床相关的组织结构至关重要.
动脉静脉循环 (AVL) 已经在各种模型中证明了在骨再生中的多功能性.
之前的研究表明,AVL在辐射后血管化组织结构方面具有能力.

研究的目的:

调查缺氧和HIF1-SDF1轴在辐射后AVL介导组织再生中的作用.
分析氧诱导因子1 (HIF1) 和 stromal-derived factor 1 (SDF1) 在被辐射的AVL骨结构中的表达.
评估辐射对这些结构中的骨质基因表达的影响.

主要方法:

在易斯老鼠骨结构中对HIF1和SDF1表达的免疫光分析.
骨质生成区分基因的实时PCR分析 (原-1,RUNX,性酸酶,骨).
在植入后2周和5周,用9Gy或15Gy对建筑物的局部辐射.

主要成果:

与对照组相比,在15Gy照射后2周观察到HIF1和SDF1的增强表达.
在5周内,骨质原生标志物的升调,包括原-1,性酸酶和骨质菌素.
在照射和AVL植入后,组织分化标志物的显著增强.

结论:

在AVL结构中的缺氧,加上高SDF1表达,促进组织分化.
这项研究支持这样一个假设,即HIF1-SDF1轴在被辐射的AVL骨结构中调节再生.
这种缺氧驱动的组织生成过程与器官生成相似,为再生策略提供了洞察力.

关键词:

动脉静脉循环的发生轴向血管化是指轴向血管化.氧气过低是因为缺氧.再生医学是一种再生医学.组织工程是组织工程.

更多相关视频

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Generation of Human Blood Vessel Organoids from Pluripotent Stem Cells

Published on: January 20, 2023

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Hypoxia

Hypoxia

Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...