通过PI3K/Akt/mTOR信号通路诱导PD-1/PD-L1轴诱导宿主免疫抑制在感染Glaesserella Parasuis的小猪身上
Jingyang Li1,2, Siyu Liu1,2, Qiaoli Dong1,2
1Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, PR China.
BMC veterinary research
|April 6, 2024
概括
在小猪中,Glaesserella parasuis感染通过改变T细胞分化和PD-1/PD-L1表达来抑制免疫系统. 蛋白质组分析揭示了与免疫反应相关的脏蛋白质的显著变化.
科学领域:
- 兽医免疫学 兽医免疫学
- 细菌病原体的产生
- 蛋白质组学是指蛋白质组学.
背景情况:
- Glaesserella parasuis会在小猪中引起Glässer病,导致呼吸系统问题和免疫抑制.
- 了解宿主对G. parasuis的免疫反应对于开发有效治疗方法至关重要.
研究的目的:
- 在小猪模型中研究G. parasuis的免疫抑制机制.
- 分析感染后脏免疫细胞群和蛋白质表达的变化.
主要方法:
- 使用G.parasuis.建立了一个小猪感染模型.
- 分析了脏组织的PD-1/PD-L1表达和免疫细胞分化 (CD3+,CD4+,CD8+T细胞,CD21+细胞).
- 使用数据独立获取 (DIA) 的蛋白质组分析在脏组织上进行.
主要成果:
- 在小猪中,G. parasuis感染改变了T细胞分化和PD-1/PD-L1表达.
- 在感染的脏组织中发现了596种差异表达蛋白 (DEPs),其中301种是上调调的,295种是下调调的.
- DEP主要与免疫反应途径有关.
结论:
- 通过调节免疫细胞群和PD-1/PD-L1表达,G. parasuis感染会诱导小猪的免疫抑制.
- 蛋白质组分析提供了对G. parasuis诱导的免疫失调背后的分子机制的见解.
- 这项研究是首次研究小猪在G. parasuis感染期间在中PD-1/PD-L1表达和蛋白质组变化的研究.
相关概念视频
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