在厄瓜多尔,抗卡巴因耐药的细菌的分子流行病学
Claudia Soria-Segarra1,2,3, Carmen Soria-Segarra4,5, Marcos Molina-Matute6
1Sosecali, Medical Services, Guayaquil, EC, 090308, Ecuador. Claudia.soria.s@gmail.com.
BMC infectious diseases
|April 6, 2024
概括
在厄瓜多尔,耐卡巴尼姆的格拉姆阴性细菌,特别是K. pneumoniae,是厄瓜多尔的主要威胁. 新型药物对氨酸-碳烯酶生产者有希望,但XDR菌株需要紧急的控制策略.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 耐卡巴尼姆抗原细菌 (CRGNB) 由于高死亡率,对全球健康构成重大威胁.
- 越来越多的CRGNB需要了解耐药性机制并评估新的治疗选择.
研究的目的:
- 在厄瓜多尔调查CRGNB的分子特征和抗菌敏感性.
- 评估特定的碳烯酶基因 (blaKPC,blaNDM,blaOXA-48,blaVIM,blaIMP,blaOXA-23,blaOXA-58,blaOXA-24/40) 的流行情况.
- 评估新型抗菌剂对这些耐药菌株的体外活性.
主要方法:
- 从厄瓜多尔的六家医院收集了95个临床隔离的卡巴胺不敏感的グラム阴性细菌.
- 通过使用梅罗胺磁盘扩散和通过修改的卡巴酶失活法产生卡巴酶的方法确认了对卡巴胺的耐药性.
- 通过磁盘扩散,Vitek 2,梯度条和微稀释来确定抗微生物敏感性;对关键的卡巴酶基因进行选.
主要成果:
- 在96.84%的隔离物中检测到碳烯酶的产生.
- 在Enterobacterales中 blaKPC 主导,在P. aeruginosa中 blaVIM 主导,在A. baumannii中 blaOXA-24/40 主导.
- 大多数隔离物表现出对常见抗生素的共同耐药性; 塞夫塔齐迪姆/阿维巴克坦和美罗/瓦博巴克坦对氨酸-碳烯酶生产物的活性.
结论:
- 厄瓜多尔的碳烯耐药性在很大程度上是由生产碳烯酶的K. pneumoniae携带blaKPC.驱动的.
- 广泛耐药 (XDR) 的P. aeruginosa和A. baumannii被确定.
- 需要紧急实施控制策略,以减轻这些高度耐药菌株的传播.
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