相关实验视频

Updated: May 2, 2026

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

In Situ Mapping of the Mechanical Properties of Biofilms by Particle-tracking Microrheology

Published on: December 4, 2015

在动态膜生物反应器中,在短暂的SRT下形成的紧且耐冲洗的网状生物膜在延长的SRT下自然消失

Ju Huang¹, Xianwei Wu², Zhongya Fan³

¹National Key Laboratory of Water Environmental Simulation and Pollution Control, Guangdong Key Laboratory of Water and Air Pollution Control, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, No 18 Ruihe Road, Guangzhou 510530, China; School of Environment and Climate, Guangdong Engineering Research Center of Water Treatment Processes and Materials, and Guangdong Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou, 510632, China.

Journal of environmental management

|April 7, 2024

概括

在动态膜生物反应器 (DMBR) 中延长固体保留时间 (SRT) 有效地生物降解固的网状生物膜. 这一过程恢复了过器的透性和流量,无需手动清洁,展示了膜生物污染管理的可持续方法.

科学领域:

环境科学环境科学
生物技术是生物技术.
水处理处理水的方法

背景情况:

膜生物污染,特别是紧的和耐冲洗的生物膜,在动态膜生物反应器 (DMBR) 中显著降低了膜性能.
延长固体保留时间 (SRT) 是一种已知的减轻生物污染的策略,但对已建立的,强大的生物膜的有效性不太清楚.

研究的目的:

在动态膜生物反应器 (DMBR) 在延长SRT (40天) 运行时,研究在短SRT (10天) 形成的紧网生物膜的生物降解和去除.
评估扩展SRT对波器液压阻力,水流回收和生物膜组成的影响.

主要方法:

从10天的SRT DMBR中直接重复使用到40天的SRT DMBR中,没有清洗,从10天的SRT DMBR中直接重复使用一个生物填充网状过器.
扫描电子显微镜 (SEM) 和共聚焦激光扫描显微镜 (CLSM) 用于分析生物膜结构和去除.
测量了液压阻力,水流量以及生物膜中的蛋白质和多糖的密度.
在污泥和生物膜中分析了定数灭细菌的丰度,使用相对丰度.

主要成果:

重复使用的网状过器从第10天开始显示引力驱动的流量,并在40天的SRT时在第27天恢复到36.7 L m-2·h-1
SEM和CLSM证实了紧的10天SRT生物薄膜的生物降解和去除,剩余的生物薄膜变得可水冲洗.
液压阻力显著降低 (4.36 × 108 至 6.97 × 107 m-1),流量完全恢复.
生物膜中的蛋白质和多糖含量大幅下降,与粘附性降低相关.
在40天的SRT反应堆中观察到较高的定量灭细菌 (例如,) 的相对丰度,可能与减少的细胞外聚合物物质和生物膜降解有关.

结论:

延长固体保留时间 (40天) 有效地生物降解并去除在DMBR中在较短的SRT (10天) 形成的紧,耐水冲洗的网状生物膜.
这种生物降解过程导致液压性能和流量完全恢复,为膜生物污染控制提供了无清洗策略.
在延长的SRT中,定数灭细菌的增加可能在观察到的细胞外聚合物物质的减少和随后的生物膜去除中发挥关键作用.

关键词:

生物降解生物降解生物膜是一种生物膜.动态膜生物反应器是一个动态膜生物反应器.细胞外聚合物质是细胞外的聚合物质.固体的保留时间固体的保留时间

更多相关视频

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

Published on: August 18, 2017

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

相关概念视频

Biofilms

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...

Microbial Mats

Microbial Mats

Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...

Bioreactor Design and Operational System

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...