收集和无氧发酵技术的验证,以测量益生菌对肠道微生物群的影响
Alexander W Thorman1, Ardythe L Morrow1, Andre Groeneveld2
1University of Cincinnati College of Medicine, Department of Environmental and Public Health Sciences, Kettering Labs, 160 Panzeca Way, Cincinnati, OH 45267, United States.
Pharmacological research
|April 7, 2024
概括
这项研究验证了分析益生菌对肠道微生物群的影响的方法. 标准便收集和冷保存在临床试验中可靠用于发酵分析.
科学领域:
- 微生物学 微生物学
- 人类肠道微生物组研究研究
- 营养科学 营养科学
背景情况:
- 益生菌饮食碳水化合物影响肠道微生物群的组成和新陈代谢.
- 了解这些影响对于评估个人健康影响至关重要.
- 目前的临床试验通常依赖于间接测量方法.
研究的目的:
- 验证用于临床研究的便收集和发酵方法.
- 建立可靠的技术来分析对人类便微生物群的益生菌影响.
- 为了能够准确地评估各种人群中的益生菌疗效.
主要方法:
- 在各种条件下从婴儿和成年人收集便样本.
- 用益生菌无氧培养样本,测量光学密度和pH值.
- 利用全基因组射击枪测序和NMR代谢学进行分析.
主要成果:
- 收集瓶类型和冷保存都没有显著改变微生物组成或代谢组的主要组成部分.
- 微生物组成的差异主要是主体驱动的.
- 代谢差异是由使用的特定发酵糖驱动的.
结论:
- 在大型临床研究中确定了一种可行和有效的方法来分析前生物发酵.
- 建议在测试前采集标准瓶子和冷保存.
- 发酵分析是一种有效的技术,用于评估 prebiotika 对人类便微生物群的影响.
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